Alpha-amylase inhibitory activity and in silico studies of in vitro sweet basil plantlets treated with chitosan and ZnO NPs

IF 2.2 3区生物学 Q4 CELL BIOLOGY In Vitro Cellular & Developmental Biology - Plant Pub Date : 2024-01-02 DOI:10.1007/s11627-023-10401-0

{"title":"Alpha-amylase inhibitory activity and in silico studies of in vitro sweet basil plantlets treated with chitosan and ZnO NPs","authors":"","doi":"10.1007/s11627-023-10401-0","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>In this study, an efficient protocol was developed to obtain multiplicated, healthy sweet basil plantlets with larger leaves and a greener color by culturing them on MS medium containing 1.0 mg/l 2iP. Chitosan and zinc oxide nanoparticles (ZnO NPs) were used as abiotic elicitors to maximize the main secondary metabolites (phenolic and flavonoid compounds and essential oil) in <em>in vitro</em> plantlets of sweet basil. Chitosan with a concentration of 4.0 mg/l recorded the maximum value of caffeic acid (1.189 mg/g DW), and 10 mg/l of ZnO NPs recorded the maximum content of rutin (0.936 mg/g DW). The plantlets cultured with 2.0 mg/l chitosan recorded the maximum percentage of essential oil (90.63%). 8,9-Dihydro-9-formyl cycloisolongifolene was the major compound (72.44%) and was detected in the plantlets treated with 2.0 mg/l chitosan. The inhibitory activity evaluation of α-amylase revealed that the maximum inhibitory activity was found in the plantlet control and those treated with 4.0 mg/l chitosan of the phenolic and flavonoid fractions. The maximum IC<sub>50</sub> (7.36 ± 0.85) was found in the plantlet extract treated with 4.0 mg/l chitosan, followed by the plantlet control, which recorded 11.53 ± 0.86. Using AutoDuck 4.2 and PyMol software, it was revealed that rutin and caffeic acid compounds (the main active components) own hydrogen binding interactions and hydrophobic binding interactions with the active site of alpha-amylase from the porcine pancreas (PPA) enzyme with good binding energy (5.74 and 5.71 kcal/mol, respectively).</p>","PeriodicalId":13293,"journal":{"name":"In Vitro Cellular & Developmental Biology - Plant","volume":"13 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"In Vitro Cellular & Developmental Biology - Plant","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11627-023-10401-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, an efficient protocol was developed to obtain multiplicated, healthy sweet basil plantlets with larger leaves and a greener color by culturing them on MS medium containing 1.0 mg/l 2iP. Chitosan and zinc oxide nanoparticles (ZnO NPs) were used as abiotic elicitors to maximize the main secondary metabolites (phenolic and flavonoid compounds and essential oil) in in vitro plantlets of sweet basil. Chitosan with a concentration of 4.0 mg/l recorded the maximum value of caffeic acid (1.189 mg/g DW), and 10 mg/l of ZnO NPs recorded the maximum content of rutin (0.936 mg/g DW). The plantlets cultured with 2.0 mg/l chitosan recorded the maximum percentage of essential oil (90.63%). 8,9-Dihydro-9-formyl cycloisolongifolene was the major compound (72.44%) and was detected in the plantlets treated with 2.0 mg/l chitosan. The inhibitory activity evaluation of α-amylase revealed that the maximum inhibitory activity was found in the plantlet control and those treated with 4.0 mg/l chitosan of the phenolic and flavonoid fractions. The maximum IC₅₀ (7.36 ± 0.85) was found in the plantlet extract treated with 4.0 mg/l chitosan, followed by the plantlet control, which recorded 11.53 ± 0.86. Using AutoDuck 4.2 and PyMol software, it was revealed that rutin and caffeic acid compounds (the main active components) own hydrogen binding interactions and hydrophobic binding interactions with the active site of alpha-amylase from the porcine pancreas (PPA) enzyme with good binding energy (5.74 and 5.71 kcal/mol, respectively).

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

用壳聚糖和氧化锌氮氧化物处理的离体甜罗勒小株的α-淀粉酶抑制活性和硅学研究

摘要本研究开发了一种有效的方法，通过在含有 1.0 毫克/升 2iP 的 MS 培养基上培养甜罗勒小植株，使其获得增殖的、健康的、叶片更大且颜色更绿的甜罗勒小植株。壳聚糖和氧化锌纳米粒子（ZnO NPs）被用作非生物诱导剂，以最大限度地提高甜罗勒离体小植株的主要次生代谢产物（酚类和类黄酮化合物以及精油）的含量。浓度为 4.0 毫克/升的壳聚糖记录了最大的咖啡酸含量（1.189 毫克/克 DW），浓度为 10 毫克/升的氧化锌氮氧化物记录了最大的芦丁含量（0.936 毫克/克 DW）。用 2.0 毫克/升壳聚糖培养的小植株精油含量最高（90.63%）。8,9-Dihydro-9-formyl cycloisolongifolene 是主要化合物（72.44%），在使用 2.0 毫克/升壳聚糖处理的小植株中被检测到。对α-淀粉酶的抑制活性评估显示，酚类和黄酮类组分的最大抑制活性出现在对照组和用 4.0 毫克/升壳聚糖处理的小植株中。用 4.0 毫克/升壳聚糖处理的小植株提取物的 IC50 值最大（7.36 ± 0.85），其次是小植株对照，为 11.53 ± 0.86。使用 AutoDuck 4.2 和 PyMol 软件分析发现，芦丁和咖啡酸化合物（主要活性成分）与猪胰腺（PPA）α-淀粉酶的活性位点具有氢结合作用和疏水结合作用，结合能良好（分别为 5.74 和 5.71 kcal/mol）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

In Vitro Cellular & Developmental Biology - Plant 生物-发育生物学

CiteScore

5.00

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Founded in 1965, In Vitro Cellular & Developmental Biology - Plant is the only journal devoted solely to worldwide coverage of in vitro biology in plants. Its high-caliber original research and reviews make it required reading for anyone who needs comprehensive coverage of the latest developments and state-of-the-art research in plant cell and tissue culture and biotechnology from around the world.