Cytokine gene polymorphism with type 2 diabetes and diabetic nephropathy in population from West India

IF 0.7 4区医学 Q4 ENDOCRINOLOGY & METABOLISM International Journal of Diabetes in Developing Countries Pub Date : 2024-01-03 DOI:10.1007/s13410-023-01301-5

Brijesh K. Dabhi, Kinnari N. Mistry, Jinal M. Thakor, Sishir Gang

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引用次数: 0

Abstract

Objective

Genetic polymorphisms of the angiogenesis, inflammatory cascade, or apoptosis genes can influence chronic complications in diabetic patients. Cytokine gene polymorphisms are considered vital in diabetes and diabetic nephropathy (DN) susceptibility. The present study evaluated the role of cytokine gene polymorphism in type 2 diabetes and diabetic nephropathy.

Methods

A total number of 648 participants comprising 180 healthy individuals, 164 type 2 diabetes mellitus patients without any complications, 148 individuals with diabetic nephropathy, and 156 with non-diabetic nephropathy were included in this study. The IL-6 (-634 C/G), IL-18 (-607 A/C), IL-4 (-590C/T), and IL-10 (-592 C/A) polymorphism were analyzed using the PCR-RFLP method, and their expression analysis was done using real-time PCR

Results

We found a significant difference in the genotype frequency of IL-6 and IL-10 in the diabetic nephropathy group compared to the control, whereas no significant difference was found in IL-18 and IL-4.

Conclusion

The IL-6 -634C/G and IL-10-592 C/A polymorphisms were found to be associated with diabetic nephropathy in the West Indian population. The higher transcript level of inflammatory cytokines in patient groups compared to the control group may suggest the essential role of inflammation in the pathogenesis of diabetes and diabetic nephropathy.

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西印度人群中细胞因子基因多态性与 2 型糖尿病和糖尿病肾病的关系

目的血管生成、炎症级联或细胞凋亡基因的遗传多态性可影响糖尿病患者的慢性并发症。细胞因子基因多态性被认为对糖尿病和糖尿病肾病（DN）的易感性至关重要。本研究评估了细胞因子基因多态性在 2 型糖尿病和糖尿病肾病中的作用。本研究共纳入 648 名参与者，包括 180 名健康人、164 名无任何并发症的 2 型糖尿病患者、148 名糖尿病肾病患者和 156 名非糖尿病肾病患者。采用 PCR-RFLP 方法分析了 IL-6（-634 C/G）、IL-18（-607 A/C）、IL-4（-590 C/T）和 IL-10（-592 C/A）的多态性，并采用实时 PCRR 方法对其表达进行了分析。结论在西印度人群中，IL-6 -634C/G和IL-10-592 C/A多态性与糖尿病肾病有关。与对照组相比，患者组中炎症细胞因子的转录水平较高，这可能表明炎症在糖尿病和糖尿病肾病的发病机制中起着重要作用。

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来源期刊

International Journal of Diabetes in Developing Countries ENDOCRINOLOGY & METABOLISM-

CiteScore

1.60

自引率

0.00%

发文量

109

审稿时长

6 months

期刊介绍： International Journal of Diabetes in Developing Countries is the official journal of Research Society for the Study of Diabetes in India. This is a peer reviewed journal and targets a readership consisting of clinicians, research workers, paramedical personnel, nutritionists and health care personnel working in the field of diabetes. Original research articles focusing on clinical and patient care issues including newer therapies and technologies as well as basic science issues in this field are considered for publication in the journal. Systematic reviews of interest to the above group of readers are also accepted.