How to measure your microscope's HPF. A critical guide for residents.

IF 4.4 Q1 PATHOLOGY PATHOLOGICA Pub Date : 2023-12-01 DOI:10.32074/1591-951X-900
Salvatore Lorenzo Renne
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Abstract

Counting stuff under the microscope is part of the duties of a surgical pathologist. Many textbooks and articles still report the surface area as the number of high-power fields (HPFs) counted. This is bad, since the area displayed by an HPF varies between two microscopes. It is therefore necessary to express the surface as mm2. This is a how to guide written for the resident who has to measure the HPF of the microscope for the first time. The Resident can either calibrate the microscope with a stage micrometer slide (a small ruler on a glass slide) or compute the surface area of the HPF using the numbers on the eyepiece and the magnification objective. for "10X/22" eyepiece and a "40X" objective, the diameter of the HPF is 22/40 = 0.55 (if no other magnification is present), and the surface is 0.238 mm2. The young resident might then ask: "How far off-target was I when I counted the number of HPFs that the chief resident declared to be correct?" Probably not that much: although legitimate in principle and correct in math, the size of the problem is often overstated since microscopes are not that different after all and because pathology is not just about counting.

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如何测量显微镜的 HPF。住院医生的重要指南。
在显微镜下计数是外科病理学家的职责之一。许多教科书和文章仍以高倍视野(HPF)的数量来报告表面积。这是不好的,因为两台显微镜的高倍视野所显示的面积是不同的。因此有必要用平方毫米来表示表面积。这是为第一次测量显微镜高倍视野的住院医生编写的指南。住院医师可以使用台式测微计载玻片(玻璃载玻片上的一把小尺子)校准显微镜,也可以使用目镜和放大倍率物镜上的数字计算 HPF 的表面积。对于 "10X/22 "目镜和 "40X "物镜,HPF 的直径为 22/40 = 0.55(如果没有其他放大倍率),表面积为 0.238 平方毫米。年轻的住院医师可能会问:"当我计算住院总医师宣布正确的 HPF 数量时,我偏离目标有多远?也许没有那么严重:虽然原则上是合理的,数学上也是正确的,但问题的严重性往往被夸大了,因为显微镜毕竟没有那么大的区别,而且病理学不仅仅是计算。
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来源期刊
PATHOLOGICA
PATHOLOGICA PATHOLOGY-
CiteScore
5.90
自引率
5.70%
发文量
108
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