Selective regulation of endophytic bacteria and gene expression in soybean by water-soluble humic materials.

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY Environmental Microbiome Pub Date : 2024-01-04 DOI:10.1186/s40793-023-00546-1
Wenqian Wang, Dongmei Li, Xiaoqian Qiu, Jinshui Yang, Liang Liu, Entao Wang, Hongli Yuan
{"title":"Selective regulation of endophytic bacteria and gene expression in soybean by water-soluble humic materials.","authors":"Wenqian Wang, Dongmei Li, Xiaoqian Qiu, Jinshui Yang, Liang Liu, Entao Wang, Hongli Yuan","doi":"10.1186/s40793-023-00546-1","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>As part of the plant microbiome, endophytic bacteria play an essential role in plant growth and resistance to stress. Water-soluble humic materials (WSHM) is widely used in sustainable agriculture as a natural and non-polluting plant growth regulator to promote the growth of plants and beneficial bacteria. However, the mechanisms of WSHM to promote plant growth and the evidence for commensal endophytic bacteria interaction with their host remain largely unknown. Here, 16S rRNA gene sequencing, transcriptomic analysis, and culture-based methods were used to reveal the underlying mechanisms.</p><p><strong>Results: </strong>WSHM reduced the alpha diversity of soybean endophytic bacteria, but increased the bacterial interactions and further selectively enriched the potentially beneficial bacteria. Meanwhile, WSHM regulated the expression of various genes related to the MAPK signaling pathway, plant-pathogen interaction, hormone signal transduction, and synthetic pathways in soybean root. Omics integration analysis showed that Sphingobium was the genus closest to the significantly changed genes in WSHM treatment. The inoculation of endophytic Sphingobium sp. TBBS4 isolated from soybean significantly improved soybean nodulation and growth by increasing della gene expression and reducing ethylene release.</p><p><strong>Conclusion: </strong>All the results revealed that WSHM promotes soybean nodulation and growth by selectively regulating soybean gene expression and regulating the endophytic bacterial community, Sphingobium was the key bacterium involved in plant-microbe interaction. These findings refined our understanding of the mechanism of WSHM promoting soybean nodulation and growth and provided novel evidence for plant-endophyte interaction.</p>","PeriodicalId":48553,"journal":{"name":"Environmental Microbiome","volume":"19 1","pages":"2"},"PeriodicalIF":6.2000,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10768371/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiome","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1186/s40793-023-00546-1","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: As part of the plant microbiome, endophytic bacteria play an essential role in plant growth and resistance to stress. Water-soluble humic materials (WSHM) is widely used in sustainable agriculture as a natural and non-polluting plant growth regulator to promote the growth of plants and beneficial bacteria. However, the mechanisms of WSHM to promote plant growth and the evidence for commensal endophytic bacteria interaction with their host remain largely unknown. Here, 16S rRNA gene sequencing, transcriptomic analysis, and culture-based methods were used to reveal the underlying mechanisms.

Results: WSHM reduced the alpha diversity of soybean endophytic bacteria, but increased the bacterial interactions and further selectively enriched the potentially beneficial bacteria. Meanwhile, WSHM regulated the expression of various genes related to the MAPK signaling pathway, plant-pathogen interaction, hormone signal transduction, and synthetic pathways in soybean root. Omics integration analysis showed that Sphingobium was the genus closest to the significantly changed genes in WSHM treatment. The inoculation of endophytic Sphingobium sp. TBBS4 isolated from soybean significantly improved soybean nodulation and growth by increasing della gene expression and reducing ethylene release.

Conclusion: All the results revealed that WSHM promotes soybean nodulation and growth by selectively regulating soybean gene expression and regulating the endophytic bacterial community, Sphingobium was the key bacterium involved in plant-microbe interaction. These findings refined our understanding of the mechanism of WSHM promoting soybean nodulation and growth and provided novel evidence for plant-endophyte interaction.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
水溶性腐殖质对大豆内生菌和基因表达的选择性调控。
背景:作为植物微生物群的一部分,内生细菌在植物生长和抗逆方面发挥着至关重要的作用。水溶性腐殖质材料(WSHM)作为一种天然、无污染的植物生长调节剂,被广泛应用于可持续农业,以促进植物和有益菌的生长。然而,水溶性腐殖质促进植物生长的机制以及共生内生菌与其宿主相互作用的证据在很大程度上仍然未知。本文采用 16S rRNA 基因测序、转录组分析和基于培养的方法来揭示其潜在机制:结果:WSHM 降低了大豆内生菌的α-多样性,但增加了细菌间的相互作用,并进一步选择性地富集了潜在的有益细菌。同时,WSHM 调节了大豆根部与 MAPK 信号通路、植物与病原菌相互作用、激素信号转导和合成途径相关的各种基因的表达。Omics 整合分析表明,在 WSHM 处理中,与发生显著变化的基因最接近的属是鞘氨醇属。接种从大豆中分离的内生Sphingobium sp. TBBS4后,通过增加della基因的表达和减少乙烯的释放,大豆的结瘤和生长得到了明显改善:所有研究结果表明,WSHM 通过选择性调控大豆基因表达和内生细菌群落来促进大豆的结瘤和生长。这些发现完善了我们对 WSHM 促进大豆拔节和生长机制的认识,并为植物与内生菌的相互作用提供了新的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
期刊最新文献
Phage-induced disturbance of a marine sponge microbiome. Exploring the biosynthesis potential of permafrost microbiomes. Soil properties drive nitrous oxide accumulation patterns by shaping denitrifying bacteriomes. Metatranscriptomics of microbial biofilm succession on HDPE foil: uncovering plastic-degrading potential in soil communities. Stochasticity-dominated rare fungal endophytes contribute to coexistence stability and saponin accumulation in Panax species.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1