Systemic and local antiinflammatory effect of magnesium chloride in experimental arthritis.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-01-04 DOI:10.1186/s42358-023-00346-8
Ana Carolina Matias Dinelly Pinto, Rodolfo de Melo Nunes, Waleska Vidal de Freitas Carvalho, Virgínia Claudia Carneiro Girão, Francisco Airton Castro Rocha
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Abstract

Objective: Despite some knowledge gaps in scientific evidence, MgCl2 is largely used for pain relief in musculoskeletal diseases. Mg salts were shown to provide analgesia postoperatively in orthopedic surgery and low Mg levels were linked to arthritis development and severity. We determined the anti-inflammatory activity of MgCl2 in an acute arthritis model.

Methods: Mice received 0.1 mg/25µL Zymosan (Zy) or saline into the knees. Joint pain was evaluated using von Frey test; cell influx, and interleukin (IL)-1 level were assessed in joint lavage at 6 h. Synovia were excised for histopathology and analysis of immunoexpression of nuclear factor kappa B (NFκB) and tumor necrosis factor (TNF)-α. Groups (n = 6/group) received either 90 mg/kg MgCl2/100 µL or saline per os (systemic) or 500 µg/25 µL MgCl2 or saline intra-articularly (i.a.) 30 min prior to Zy.

Results: MgCl2 given either systemically or locally significantly reduced cell influx (p = 0.0012 and p = 0.0269, respectively), pain (p = 0.0005 and p = 0.0038, respectively), and intra-articular IL-1 level (p = 0.0391), as compared to saline. Systemic MgCl2 significantly decreased NFκB (p < 0.05) immmunoexpression, as compared to saline.

Conclusion: MgCl2 given systemically or locally displayed anti-inflammatory activity in a severe acute arthritis model reducing cell influx, pain, and cytokine release. MgCl2 operates at least partially via inhibiting NFκB activation. This is the first in vivo demonstration that MgCl2 decreases cytokine release in arthritis, prompting reduction of inflammation and pain relief.

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氯化镁在实验性关节炎中的全身和局部抗炎作用。
目的:尽管在科学证据方面存在一些知识空白,但氯化镁在很大程度上被用于缓解肌肉骨骼疾病的疼痛。在骨科手术中,镁盐可提供术后镇痛,而镁含量低与关节炎的发展和严重程度有关。我们测定了氯化镁在急性关节炎模型中的抗炎活性:小鼠膝关节接受 0.1 mg/25µL Zymosan (Zy) 或生理盐水注射。滑膜切除后进行组织病理学检查,并分析核因子卡巴B(NFκB)和肿瘤坏死因子(TNF)-α的免疫表达。各组(n = 6/组)在 Zy 前 30 分钟接受 90 mg/kg MgCl2/100 µL 或生理盐水全身注射,或 500 µg/25 µL MgCl2 或生理盐水关节内注射:与生理盐水相比,全身或局部注射氯化镁可显著减少细胞流入(分别为 p = 0.0012 和 p = 0.0269)、疼痛(分别为 p = 0.0005 和 p = 0.0038)和关节内 IL-1 水平(p = 0.0391)。全身注射氯化镁能明显降低 NFκB(p 结论:氯化镁能明显降低关节内 IL-1 水平(p = 0.0391):在严重急性关节炎模型中,全身或局部注射氯化镁具有抗炎活性,可减少细胞流入、疼痛和细胞因子释放。氯化镁至少部分通过抑制 NFκB 激活发挥作用。这是首次在体内证明氯化镁能减少关节炎细胞因子的释放,从而减轻炎症和疼痛。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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