Race specific and non-specific resistance to Magnaporthe oryzae and QTL mapping in wild introgression lines using the standard differential system

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences Tropical Plant Pathology Pub Date : 2024-01-05 DOI:10.1007/s40858-023-00614-4
Divya Balakrishnan, Yoshimichi Fukuta, Sarla Neelamraju
{"title":"Race specific and non-specific resistance to Magnaporthe oryzae and QTL mapping in wild introgression lines using the standard differential system","authors":"Divya Balakrishnan, Yoshimichi Fukuta, Sarla Neelamraju","doi":"10.1007/s40858-023-00614-4","DOIUrl":null,"url":null,"abstract":"<p>Blast disease caused by <i>Magnaporthe oryzae</i> is one of the major challenges to rice crop production worldwide. Wild and related species are potential source of novel alleles or genes for crop improvement especially for biotic stress resistance. This study was designed to characterize blast resistance in a set of wild introgression lines developed in the background of Indian mega rice variety Swarna. The introgression lines (INLs) under this study showed spectra of resistance across the different blast isolates inoculated. Monogenic differential varieties with known specific <i>Pi</i> genes and the susceptible checks were used in phenotyping. This study confirmed the usefulness of the differential system consisting of monogenic varieties and differential blast isolates for the systematic characterization of resistance in novel germplasm and to understand the genetic architecture of blast resistance. Twelve race specific quantitative trait loci (QTL) for partial resistance to disease were detected in these wild introgression lines from <i>O.nivara</i> using composite interval mapping. Of these, 10 were showing resistance to only one isolate each, while QTL detected in chromosome 3 showed resistance against isolates JPF514 and PHL16. While most of the QTLs mapped to previously reported defense related genes whereas two QTLs <i>qBL2.2</i> and <i>qBL5.1</i> between RM106—RM5460 and RM5140—RM289 with PVE% of 12.28 and 12.48 respectively are novel with no known blast resistance related genes reported within the locus. This study helps to provide developing the basis for future investigations on race specific or broad-spectrum resistance in rice and related crop species.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-023-00614-4","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

Blast disease caused by Magnaporthe oryzae is one of the major challenges to rice crop production worldwide. Wild and related species are potential source of novel alleles or genes for crop improvement especially for biotic stress resistance. This study was designed to characterize blast resistance in a set of wild introgression lines developed in the background of Indian mega rice variety Swarna. The introgression lines (INLs) under this study showed spectra of resistance across the different blast isolates inoculated. Monogenic differential varieties with known specific Pi genes and the susceptible checks were used in phenotyping. This study confirmed the usefulness of the differential system consisting of monogenic varieties and differential blast isolates for the systematic characterization of resistance in novel germplasm and to understand the genetic architecture of blast resistance. Twelve race specific quantitative trait loci (QTL) for partial resistance to disease were detected in these wild introgression lines from O.nivara using composite interval mapping. Of these, 10 were showing resistance to only one isolate each, while QTL detected in chromosome 3 showed resistance against isolates JPF514 and PHL16. While most of the QTLs mapped to previously reported defense related genes whereas two QTLs qBL2.2 and qBL5.1 between RM106—RM5460 and RM5140—RM289 with PVE% of 12.28 and 12.48 respectively are novel with no known blast resistance related genes reported within the locus. This study helps to provide developing the basis for future investigations on race specific or broad-spectrum resistance in rice and related crop species.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
对 Magnaporthe oryzae 的种族特异性和非特异性抗性以及利用标准差异系统在野生引种品系中绘制 QTL 图谱
由 Magnaporthe oryzae 引起的白叶枯病是全球水稻作物生产面临的主要挑战之一。野生及相关物种是作物改良(尤其是抗生物胁迫)的新型等位基因或基因的潜在来源。本研究旨在描述以印度大型水稻品种 Swarna 为背景开发的一组野生引种系的稻瘟病抗性。本研究中的引种品系(INLs)对接种的不同稻瘟病分离物均表现出抗性。具有已知特异性 Pi 基因的单基因差异品种和易感对照被用于表型分析。这项研究证实了由单基因品种和不同稻瘟病分离株组成的差异系统对系统鉴定新种质的抗性和了解稻瘟病抗性遗传结构的有用性。利用复合间隔作图法,在这些来自 O.nivara 的野生引种品系中检测到了 12 个种族特异性抗病性的数量性状位点(QTL)。其中,10 个基因位点只对一种分离物具有抗性,而在第 3 号染色体上检测到的 QTL 则对 JPF514 和 PHL16 分离物具有抗性。大多数 QTL 映射到以前报道过的抗病相关基因,而 RM106-RM5460 和 RM5140-RM289 之间的两个 QTL qBL2.2 和 qBL5.1 是新的,PVE% 分别为 12.28 和 12.48,该基因座内没有报道过已知的抗瘟相关基因。这项研究有助于为今后研究水稻及相关作物物种的种族特异性或广谱抗性奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
期刊最新文献
Genetic variability and population structure of Pseudocercospora griseola isolates in the Black Sea region of Türkiye Identification of the causal agent of Guar leaf blight and development of a semi-automated method to quantify disease severity Genomic analysis identifies five pathogenic bacterial species in Argentinian wheat Detached leaf assay-based screening of anthracnose resistant wild strawberry genotypes Spatiotemporal dynamics of Fusarium wilt of banana caused by Subtropical Race 4
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1