{"title":"Ethylamine as new derivatization reagent differentiating reducing from non-reducing saccharides","authors":"","doi":"10.1007/s00764-023-00266-9","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>Typical derivatization reagents for saccharides in high-performance thin-layer chromatography, like 2-naphthol sulfuric acid, aniline diphenylamine orthophosphoric acid, or p-aminobenzoic acid, generally detect both reducing and non-reducing saccharides. A new reagent was found with ethylamine, specifically reacting with reducing saccharides on normal-phase silica gel plates, resulting in strongly fluorescent zones after heating the plate at 150 °C for 15 min. In contrast, non-reducing saccharides generally did not reveal fluorescent signals tested with 26 different saccharides. Optimal chromatographic separation was achieved with a mixture of 2-propyl acetate, methanol, and water with 1 mg/mL natural product reagent A when the plate was twofold developed. The high sensitivity of the ethylamine derivatization was shown with mean limits of detection and quantification of 10 and 30 ng per zone, respectively, calculated by different methods for selected mono- and disaccharides. The developed method has exemplarily been used for the digestion control of starch by α-amylase, the determination of lactose in lactose-free milk, and for the quantitative and qualitative study of honey. The analysis of honey gave an excellent example of the advantageous consecutive derivatization with ethylamine and aniline diphenylamine orthophosphoric acid reagent as reagent sequence to detect the coelution of reducing and non-reducing saccharides.</p>","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jpc-journal of Planar Chromatography-modern Tlc","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00764-023-00266-9","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Typical derivatization reagents for saccharides in high-performance thin-layer chromatography, like 2-naphthol sulfuric acid, aniline diphenylamine orthophosphoric acid, or p-aminobenzoic acid, generally detect both reducing and non-reducing saccharides. A new reagent was found with ethylamine, specifically reacting with reducing saccharides on normal-phase silica gel plates, resulting in strongly fluorescent zones after heating the plate at 150 °C for 15 min. In contrast, non-reducing saccharides generally did not reveal fluorescent signals tested with 26 different saccharides. Optimal chromatographic separation was achieved with a mixture of 2-propyl acetate, methanol, and water with 1 mg/mL natural product reagent A when the plate was twofold developed. The high sensitivity of the ethylamine derivatization was shown with mean limits of detection and quantification of 10 and 30 ng per zone, respectively, calculated by different methods for selected mono- and disaccharides. The developed method has exemplarily been used for the digestion control of starch by α-amylase, the determination of lactose in lactose-free milk, and for the quantitative and qualitative study of honey. The analysis of honey gave an excellent example of the advantageous consecutive derivatization with ethylamine and aniline diphenylamine orthophosphoric acid reagent as reagent sequence to detect the coelution of reducing and non-reducing saccharides.
期刊介绍：
JPC - Journal of Planar Chromatography - Modern TLC is an international journal devoted exclusively to the publication of research papers on analytical and preparative planar chromatography. The journal covers all fields of planar chromatography, on all kinds of stationary phase (paper, layer, gel) and with various modes of migration of the mobile phase (capillary action or forced flow).
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
