Bioactive And Cytotoxic Potentials of the Extract Fractions of Strophanthus Hispidus on Neuroblastoma (SH-SY5Y) Cell Line Model of Alzheimer’s Disease

P. A. Akinyemi, O. A. Adebesin, O. M. Arotiba, A. Adewole, J. E. Okpuzor
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Abstract

Alzheimer disease (AD) is a neurodegenerative disorder that affect memory and cognitive function. Currently, the available agents used in managing this condition are associated with severe side effects and have limited efficacy in reducing or modulating events associated with AD. Hence, there is a need for an alternative therapy with fewer side effects and better efficacy. Strophantus hispidus (SH) is a plant product widely used in Africa for its therapeutic potential.  This study was conducted to assess the bioactive constituent of the SH extract fractions, as well as the cytotoxic potential of these fractions.  The root of SH was pulverized and extracted with 80% methanol. The crude extract was then fractionated with Chloroform, Hexane, and Ethylacetate. The extract and fractions were then subjected to Gas Chromatography-Mass Spectrometry (GC-MS).  SH-SY5Y cell line model of AD was treated with graded concentrations (9.77 – 5000 µg/mL) of the SH extract and fractions for 24 hours. Cell viability assay was performed using WST-8 proliferation assay. The EC50 was determined to be 48.4 μg/mL, 15.16 μg/mL, and 151 μg/mL for cells treated with Chloroform, Ethylacetate, and Hexane fractions of Strophantus hispidus respectively. The selectivity index (SI) greater than 3 of the hexane and ethylacetate fractions were determined to be safe, less toxic, and effective in improving cellular growth.
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石蒜提取物对阿尔茨海默病神经母细胞瘤(SH-SY5Y)细胞系模型的生物活性和细胞毒性潜力
阿尔茨海默病(AD)是一种影响记忆和认知功能的神经退行性疾病。目前,用于治疗这种疾病的药物都有严重的副作用,而且在减少或调节与阿尔茨海默病相关的事件方面疗效有限。因此,需要一种副作用小、疗效好的替代疗法。Strophantus hispidus(SH)是一种在非洲广泛使用的植物产品,具有治疗潜力。 本研究旨在评估糙叶芹提取物馏分的生物活性成分以及这些馏分的细胞毒性潜力。 将 SH 的根部粉碎并用 80% 的甲醇提取。然后用氯仿、正己烷和乙酸乙酯对粗提取物进行分馏。然后对提取物和馏分进行气相色谱-质谱分析(GC-MS)。 用不同浓度(9.77 - 5000 µg/mL)的 SH 提取物和馏分处理 AD 的 SH-SY5Y 细胞株模型 24 小时。使用 WST-8 增殖试验进行细胞活力检测。氯仿、乙酸乙酯和正己烷馏分处理细胞的 EC50 分别为 48.4 μg/mL、15.16 μg/mL 和 151 μg/mL。经测定,正己烷和乙酸乙酯馏分的选择性指数(SI)大于 3,因此安全、毒性低,并能有效改善细胞生长。
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