Biofilm Inhibitory Effects of Lactobacillus Spp Against Streptomycin-resistant Uropathogenic Escherichia Coli

Nwanekwu Kenneth Emeka
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Abstract

The biofilm inhibitory effects of Lactobacillus spp against Streptomycin-resistant uropathogenic Escherichia coli (UPEC) were evaluated using the crystal violet test method. Lactobacillus spp were isolated from milk samples while fifty strains of Uropathogenic Escherichia coli were isolated from urine samples from Urinary Tract Infection patients attending Federal Medical Centre (FMC) Owerri, Nigeria. Ten of the E. coli strains resistant to streptomycin antibiotics were screened for their susceptibility to antibiofilm effect of Lactobacillus secondary metabolites extracts. From the result obtained, only one of the E. coli strains was susceptible while nine strains were resistant. This result shows clearly that the metabolite extracts from Lactobacillus sp were not effective in the antibiofilm activity of the E. coli strains and thus not a good candidate for the management of UTI caused by E. coli.
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乳酸杆菌对耐链霉素尿路致病性大肠杆菌的生物膜抑制作用
使用水晶紫试验法评估了乳酸杆菌对耐链霉素尿路致病性大肠杆菌(UPEC)生物膜的抑制作用。乳酸杆菌是从牛奶样本中分离出来的,而 50 株尿路致病性大肠杆菌则是从尼日利亚奥韦里联邦医疗中心(FMC)就诊的尿路感染患者的尿液样本中分离出来的。对其中对链霉素抗生素耐药的 10 株大肠杆菌进行了筛选,以确定它们对乳酸杆菌次级代谢产物提取物抗生物膜效应的敏感性。从结果来看,只有一株大肠杆菌具有易感性,而九株大肠杆菌具有抗药性。这一结果清楚地表明,乳酸杆菌次生代谢物提取物对大肠杆菌菌株的抗生物膜活性无效,因此不适合用于治疗由大肠杆菌引起的UTI。
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