A laboratory Evaluation of Antibacterial Activity of Commiphora myrrha Gum Resins from Kingdom of Saudi Arabia 2023

Y. Elhaj
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Abstract

Background: Today therapeutic plants are regarded as an essential part of human life, the study of antimicrobial activities of plants involves the care of the possible practice of these ordinary crops Objective: The main goal of the present work is to assess the antibacterial activity of Commiphora myrrah on pathogenic bacteria. Methods: The experimental study was conducted between April and May 2023. Fresh Commiphora myrrah gums used in my work were collected from Aqiq town, Saudi Arabia. Commiphora myrrah gums were effectively extracted by using cool and hot water for 48 hrs. as traditional uses . Five strains of bacteria were used. Minimum Inhibitory Concentration was determined by using the agar well diffusion method as the method reported by Saadabi et al. (2012). We used three drops of overnight cultured bacteria. Each extract; hot water and cool water was verified in contradiction of the five strains of the organism using the agar diffusion method (Groove and Randall, 1955) and measured the zones of inhibition. Results: In this study resin Commiphora myrrah was selected to estimate their antimicrobial activities against five isolated bacteria (one strain Gram-positive and 4 strains Gram-negative). Our findings similarly exposed that C. myrrah hot extract gives advanced antimicrobial activities on tested isolate strains.Despite, there were similar efficiency of C. myrrah and the used antibiotics on almost all tested microorganisms, sometimes C.myrrah has more efficiency than some antibiotics against some tested bacterial strains.Cool extracts of C.myrrah have less efficiency against all tested strains.
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2023 年沙特阿拉伯王国薄荷树胶抗菌活性的实验室评估
背景:如今,食疗植物已被视为人类生活中不可或缺的一部分,对植物抗菌活性的研究涉及到对这些普通作物的可能做法的关注:本研究的主要目的是评估茜草对病原菌的抗菌活性。方法:实验研究于 2023 年 4 月至 5 月间进行。我在工作中使用的新鲜荚蒾树胶是从沙特阿拉伯的 Aqiq 镇采集的。按照传统方法,用凉水和热水浸泡 48 小时,有效地提取了荚膜树胶。使用了五种菌株。按照 Saadabi 等人(2012 年)报告的方法,使用琼脂井扩散法测定最小抑菌浓度。我们使用了三滴隔夜培养的细菌。使用琼脂扩散法(Groove 和 Randall,1955 年)验证了每种提取物、热水和凉水对五种菌株的抑制作用,并测量了抑制区。结果在这项研究中,我们选择了荚蒾树脂来评估其对五种分离细菌(一株革兰氏阳性菌和四株革兰氏阴性菌)的抗菌活性。我们的研究结果同样表明,茜草热萃取物对测试分离菌株具有较强的抗菌活性。尽管茜草和所用抗生素对几乎所有测试微生物的抗菌效率相似,但有时茜草对某些测试细菌菌株的抗菌效率比某些抗生素更高,茜草冷萃取物对所有测试菌株的抗菌效率较低。
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