Major Histocompatibility Complex-DQA Variants Analysis: Rich Repertoire with Selection Advantages and Disadvantages

Abstract

Insulin dependent diabetes mellitus, an autoimmune disorder in human being, is associated with MHC class-II, which reacts with auto peptide released by MHC class-II. HLA-DQ alleles (DQA1 and DQB1) are revealed to be linked with type 1 diabetes in humans. The DQ alleles passed from parents to the offspring predisposes them with risk of type I diabetes. In present study, DQ variants (DQA1 and DQA2) were amplified and sequenced with product size 826 bp (DQA1) and 865 bp (DQA2) from sheep’s blood. Sequences were insilico characterized for percent identity, phylogeny, and protein property. Phylogeny analysis suggests a rich repertoire of DQA alleles in sheep. Ramachandran plot analysis had shown the residues are occurring under the core region of both the DQ loci with 90.8% and 92.1% of residues lie in the favored region in DQA1 and DQA2 loci, respectively.