Circulating miR-199a and long noncoding-RNA ANRIL as Promising Diagnostic Biomarkers for Inflammatory Bowel Disease.

IF 4.5 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Inflammatory Bowel Diseases Pub Date : 2024-09-03 DOI:10.1093/ibd/izad210

Randa Erfan, Olfat G Shaker, Mahmoud A F Khalil, Fatma A M Mahmoud, Mohamed S Gomaa, Abeer K Abu-El-Azayem, Othman M Zaki, Azza M Ahmed, Amira Samy, Asmaa Mohammed

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Abstract

Background & aims: Inflammatory bowel disease (IBD), involving both Crohn's disease (CD) and ulcerative colitis (UC), represents a chronic, immune-mediated inflammatory disease due to an uncontrolled, ongoing inflammatory response to intestinal bacteria in those with genetic susceptibility. MicroRNA (miRNA) extrusion from relevant remote organs or tissues is reflected in the expression of miRNAs in serum and plasma. Both UC and CD patients had higher blood levels of expressed miR-199a. Long noncoding RNA (lncRNA) ANRIL is a proinflammatory gene that mediates nuclear factor κB to play a role in inflammatory diseases, such as IBD. The aim of the current study is to investigate the potential role of both miR-199a and ANRIL in diagnosing IBD in adult patients.

Methods: Sixty-seven IBD patients diagnosed clinically, radiologically, endoscopically, and histologically were included in this prospective cohort study. Participants were classified into 3 groups: the UC group (n = 35), the CD group (n = 32), and the control group (n = 30). Demographics, history taking, laboratory characteristics, and treatments were recorded. Tumor necrosis factor α, miR-199a, and ANRIL were measured.

Results: The findings suggested that miR-199a and ANRIL might be associated with the occurrence or progression of IBD because both genes were substantially expressed in the peripheral blood of patients with this condition. Receiver-operating characteristic curve analysis indicated that the detection of miR-199a and ANRIL had a predictive sensitivity of 62.9% and 88.6% and a specificity of 70.7% and 96.7% for the occurrence of UC cases, respectively, and a predictive sensitivity of 72.4% and 46.9% and a specificity of 96.7% and 34.7% for the occurrence of CD cases, respectively.

Conclusions: Both miR-199a and ANRIL are abundant in the sera of IBD adult Egyptian patients (UC and CD). Both can represent a noninvasive marker for early disease diagnosis.

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循环 miR-199a 和长非编码 RNA ANRIL 作为炎症性肠病的有望诊断生物标记物

背景与目的：炎症性肠病（IBD）包括克罗恩病（CD）和溃疡性结肠炎（UC），是一种免疫介导的慢性炎症性疾病，是由于遗传易感人群对肠道细菌的不可控、持续的炎症反应所致。血清和血浆中 miRNA 的表达反映了相关远端器官或组织的微 RNA（miRNA）挤出。UC 和 CD 患者血液中表达的 miR-199a 水平都较高。长非编码 RNA（lncRNA）ANRIL 是一种促炎症基因，可介导核因子κB 在炎症性疾病（如 IBD）中发挥作用。本研究旨在探讨 miR-199a 和 ANRIL 在诊断成年 IBD 患者中的潜在作用：这项前瞻性队列研究共纳入了 67 名经临床、放射学、内镜和组织学诊断的 IBD 患者。参与者分为三组：UC 组（35 人）、CD 组（32 人）和对照组（30 人）。人口统计学、病史采集、实验室特征和治疗方法均有记录。对肿瘤坏死因子α、miR-199a和ANRIL进行了测定：结果：研究结果表明，miR-199a和ANRIL可能与IBD的发生或发展有关，因为这两个基因在IBD患者的外周血中大量表达。接收者工作特征曲线分析表明，检测 miR-199a 和 ANRIL 对 UC 病例发生的预测敏感性分别为 62.9% 和 88.6%，特异性分别为 70.7% 和 96.7%；对 CD 病例发生的预测敏感性分别为 72.4% 和 46.9%，特异性分别为 96.7% 和 34.7%：结论：miR-199a和ANRIL在埃及成年IBD患者（UC和CD）的血清中含量丰富。两者都可以作为早期疾病诊断的非侵入性标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Inflammatory Bowel Diseases 医学-胃肠肝病学

CiteScore

9.70

自引率

6.10%

发文量

462

审稿时长

1 months

期刊介绍： Inflammatory Bowel Diseases® supports the mission of the Crohn''s & Colitis Foundation by bringing the most impactful and cutting edge clinical topics and research findings related to inflammatory bowel diseases to clinicians and researchers working in IBD and related fields. The Journal is committed to publishing on innovative topics that influence the future of clinical care, treatment, and research.