Indukce organogeneze a multiplikace korkovníku amurského (Phellodendron amurense Rupr.) v in vitro podmínkách

Abstract

Amurk cork tree (Phellodendron amurense Rupr.) is known for its anti-inflammatory, antipyretic, cholagogue, and antibacterial properties, and it is also a potential source of industrial cork. Shoot organogenesis and subsequent plant multiplication of Amur cork tree were established from mature seeds of trees growing on research plots in the Czech Republic. Germination percentage was increased by mechanical disruption of the seed coat, the highest number of germinated plants was achieved within 8–10 weeks of culture on MS medium containing 2 mg.l-1 BAP and 0,2 mg.l-1 NAA. Within 4 weeks of transfer to the multiplication medium containing 2 mg.l-1 BAP and 0,2 mg.l-1 NAA, chlorosis and reduced growth were observed in all cultured microcuttings. Lowering the BAP concentration to 0,4 mg.l-1 led to a resumption of growth, the newly emerging shoots and leaves were vital and the multiplication rate was around 90%. Our developed plant regeneration method may be useful in future studies on ex vitro establishment of in vitro derived plants.