Scalp microbiome composition changes and pathway evaluations due to effective treatment with Piroctone Olamine shampoo

IF 2.7 4区医学 Q2 DERMATOLOGY International Journal of Cosmetic Science Pub Date : 2024-01-09 DOI:10.1111/ics.12933

Ping Hu, Jim Henry, Jay P. Tiesman, Mirjana Parlov, Rob Bacon, Duane Charbonneau, Arvind Venkataraman, Kathryn C. S. Locker, Holly Krigbaum, Jim Schwartz

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Abstract

Objective

To characterize the scalp microbial composition, function, and connection to dandruff severity using a metagenomics approach and to understand the impact of a Piroctone Olamine containing anti-dandruff shampoo on the scalp microbiome.

Methods

Shotgun metagenomics was used to characterize the composition of the scalp microbiomes from 94 subjects with and without clinically defined dandruff. Furthermore, the microbiome of the scalps of 100 dandruff sufferers before and after 3 weeks of treatment with either control or anti-dandruff shampoo containing 0.5% Piroctone Olamine (PO) was characterized and compared to identify microorganisms associated with the dandruff condition and the associated pathways and processes that may contribute to PO's effect on scalp microbiome.

Results

A higher relative abundance of Malassezia restricta and Staphylococcus capitis and a lower abundance of Cutibacterium acnes were associated with the dandruff scalps relative to the no-dandruff scalps. A 3-week PO shampoo treatment reduced the relative abundance of Malassezia species and Staphylococcus capitis and increased the relative abundance of Cutibacterium acnes. This change to the scalp microbiome composition is consistent with a return to a healthy no-dandruff microbiome and improved clinical signs and symptoms as measured by adherent scalp flaking score (ASFS) compared with the control shampoo. Functional genomics analysis showed that the PO shampoo treatment reduced oxidative stress-associated genes and decreased the abundance of protease, urease, and lipase genes. These changes correlated positively to improvements in dandruff severity. PO treatment favourably shifted scalp microbiomes in dandruff subjects toward the no-dandruff state.

Conclusion

Our results suggest that part of the aetiology of dandruff can be attributed to dysbiosis of the scalp microbiome. PO treatment can restore a healthier microbiome, reducing oxidative stress and promoting better scalp health.

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头皮微生物群组成的变化以及对使用吡罗酮欧拉敏洗发水进行有效治疗后的途径评估。

目的采用元基因组学方法描述头皮微生物的组成、功能以及与头皮屑严重程度的关系，并了解含吡罗辛酯的去屑洗发水对头皮微生物组的影响：方法：采用霰弹枪元基因组学分析了94名患有和未患有临床定义的头皮屑的受试者的头皮微生物组的组成特征。此外，还对 100 名头皮屑患者在使用对照组或含有 0.5% 吡罗辛酮乌拉敏（PO）的去屑洗发水治疗 3 周之前和之后的头皮微生物组进行了表征和比较，以确定与头皮屑状况相关的微生物，以及可能导致吡罗辛酮乌拉敏对头皮微生物组产生影响的相关途径和过程：结果：与无头皮屑的头皮相比，有头皮屑的头皮中马拉色菌和头皮葡萄球菌的相对丰度较高，而痤疮杆菌的丰度较低。为期 3 周的 PO 洗发水治疗降低了马拉色菌和头皮葡萄球菌的相对丰度，增加了痤疮角质杆菌的相对丰度。与对照组洗发水相比，头皮微生物群组成的这种变化与恢复健康的无头屑微生物群和改善临床症状和体征（以头皮附着性脱屑评分（ASFS）来衡量）相一致。功能基因组学分析表明，PO 洗发水处理减少了氧化应激相关基因，降低了蛋白酶、尿素酶和脂肪酶基因的丰度。这些变化与头皮屑严重程度的改善呈正相关。PO治疗可使头皮屑受试者的头皮微生物组向无头皮屑状态转变：我们的研究结果表明，头皮屑的部分病因可归咎于头皮微生物组的菌群失调。PO 治疗可以恢复更健康的微生物群，减少氧化应激，促进头皮健康。

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来源期刊

International Journal of Cosmetic Science DERMATOLOGY-

CiteScore

4.60

自引率

4.30%

发文量

期刊介绍： The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.