Male rat hypothalamic extraretinal photoreceptor Opsin3 is sensitive to osmotic stimuli and light

IF 3.3 4区 医学 Q2 ENDOCRINOLOGY & METABOLISM Journal of Neuroendocrinology Pub Date : 2024-01-09 DOI:10.1111/jne.13363
Soledad Bárez-López, Paul Bishop, Daniel Searby, David Murphy, Michael P. Greenwood
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Abstract

The light-sensitive protein Opsin 3 (Opn3) is present throughout the mammalian brain; however, the role of Opn3 in this organ remains unknown. Since Opn3 encoded mRNA is modulated in the supraoptic and paraventricular nucleus of the hypothalamus in response to osmotic stimuli, we have explored by in situ hybridization the expression of Opn3 in these nuclei. We have demonstrated that Opn3 is present in the male rat magnocellular neurones expressing either the arginine vasopressin or oxytocin neuropeptides and that Opn3 increases in both neuronal types in response to osmotic stimuli, suggesting that Opn3 functions in both cell types and that it might be involved in regulating water balance. Using rat hypothalamic organotypic cultures, we have demonstrated that the hypothalamus is sensitive to light and that the observed light sensitivity is mediated, at least in part, by Opn3. The data suggests that hypothalamic Opn3 can mediate a light-sensitive role to regulate circadian homeostatic processes.

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雄性大鼠下丘脑视网膜外光感受器 Opsin3 对渗透刺激和光敏感。
光敏蛋白Opsin 3(Opn3)存在于整个哺乳动物大脑中;然而,Opn3在该器官中的作用仍然未知。由于Opn3编码的mRNA在下丘脑视上核和室旁核对渗透压刺激有调节作用,因此我们通过原位杂交研究了Opn3在这些核中的表达。我们已经证明,Opn3存在于雄性大鼠表达精氨酸血管加压素或催产素神经肽的大细胞神经元中,并且Opn3在两种神经元类型中都会随着渗透压刺激而增加,这表明Opn3在两种细胞类型中都起作用,并且可能参与调节水平衡。利用大鼠下丘脑器官型培养物,我们证明了下丘脑对光的敏感性,而且观察到的光敏感性至少部分是由Opn3介导的。这些数据表明,下丘脑Opn3可以介导光敏作用,调节昼夜节律平衡过程。
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来源期刊
Journal of Neuroendocrinology
Journal of Neuroendocrinology 医学-内分泌学与代谢
CiteScore
6.40
自引率
6.20%
发文量
137
审稿时长
4-8 weeks
期刊介绍: Journal of Neuroendocrinology provides the principal international focus for the newest ideas in classical neuroendocrinology and its expanding interface with the regulation of behavioural, cognitive, developmental, degenerative and metabolic processes. Through the rapid publication of original manuscripts and provocative review articles, it provides essential reading for basic scientists and clinicians researching in this rapidly expanding field. In determining content, the primary considerations are excellence, relevance and novelty. While Journal of Neuroendocrinology reflects the broad scientific and clinical interests of the BSN membership, the editorial team, led by Professor Julian Mercer, ensures that the journal’s ethos, authorship, content and purpose are those expected of a leading international publication.
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