TGF-β1 Mediates the EndoMt in High Glucose-Treated Human Retinal Microvascular Endothelial Cells.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-05-01 Epub Date: 2024-01-08 DOI:10.1080/08820538.2023.2300806

Jia Ying, Peipei Wang, Xiao Jin, Li Luo, Keshuang Lai, Jun Li

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Abstract

The purpose of our study was to investigate the role of TGF-β1 in the endothelial-to-mesenchymal transition (EndoMT) and fibrosis in high glucose (HG)-treated human retinal microvascular endothelial cells (HRMECs). HRMECs were cultured not only under normal glucose (NG) conditions with or without TGF-β1, but also under HG conditions with or without the TGF-β1 inhibitor SB431542. The expression of TGF-β1 was detected by real time-PCR and enzyme-linked immunosorbent assay. Morphological changes and migration of the HRMECs were observed using electron microscopy and scratch-wound assay. Endothelial markers, such as CD31 and vascular endothelial (VE)-cadherin, and the acquisition of fibrotic markers, such as alpha smooth muscle actin (α-SMA) and fibroblast-specific protein-1 (FSP-1), were determined by immunofluorescent staining and western blot. The level of TGF-β1 was significantly upregulated in HG-treated HRMECs. And HG stimulation promoted obvious morphological changes and the migration ability in HRMECs. Our results also demonstrated increased expression of α-SMA and FSP-1, and decreased expression of CD31 and VE-cadherin, in HG-treated HRMECs. These EndoMT-related changes were promoted by TGF-β1 and abrogated by SB431542. The results of this study demonstrated the important role of TGF-β1 in HG-induced vitreoretinal fibrosis. EndoMT is likely to be involved in the associated effects.

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TGF-β1 在高血糖处理的人视网膜微血管内皮细胞中介导 EndoMt。

我们的研究旨在探讨 TGF-β1 在高糖（HG）处理的人视网膜微血管内皮细胞（HRMECs）的内皮细胞向间质转化（EndoMT）和纤维化中的作用。不仅在正常葡萄糖（NG）条件下培养视网膜微血管内皮细胞（HRMECs），而且在HG条件下培养视网膜微血管内皮细胞（HRMECs），无论是否使用TGF-β1抑制剂SB431542。TGF-β1 的表达通过实时聚合酶链式反应（real time-PCR）和酶联免疫吸附试验（enzyme-linked immunosorbent assay）进行检测。使用电子显微镜和划痕伤口试验观察了 HRMECs 的形态变化和迁移。通过免疫荧光染色和免疫印迹法测定了CD31和血管内皮（VE）-cadherin等内皮标志物以及α-平滑肌肌动蛋白（α-SMA）和成纤维细胞特异性蛋白-1（FSP-1）等纤维化标志物的获得情况。经HG处理的HRMECs的TGF-β1水平明显上调。HG刺激促进了HRMECs明显的形态变化和迁移能力。我们的研究结果还表明，在经 HG 处理的 HRMECs 中，α-SMA 和 FSP-1 的表达增加，CD31 和 VE-cadherin 的表达减少。TGF-β1 促进了这些与内切酶相关的变化，而 SB431542 则抑制了这些变化。该研究结果表明了 TGF-β1 在 HG 诱导的玻璃体视网膜纤维化中的重要作用。EndoMT可能参与了相关影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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