Association between Genetic Polymorphism of The lncRNA MIAT rs1894720 with Ischemic Stroke Risk and lncRNA MIAT Expression Levels in The Blood after An Ischemic Stroke: A Case-Control Study.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-12-31 DOI:10.22074/cellj.2023.2003573.1315

Tahereh Asadabadi, Mohammad Javad Mokhtari, Mahnaz Bayat, Anahid Safari, Afshin Borhani-Haghighi

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Abstract

Objective: Genetic aspects can play an essential role in the occurrence and development of ischemic stroke (IS). Rs1894720 polymorphism is one of the eight single nucleotide polymorphisms (SNPs) in the long non-coding RNA (lncRNA) myocardial infarction-associated transcript (MIAT) locus. The aim of study is the lncRNA MIAT rs1894720 polymorphism decreases IS risk by reducing lncRNA MIAT expression.

Materials and methods: In this case-control study, we studied 232 Iranian patients and 232 controls. The blood samples were collected from patients admitted at different times after stroke symptoms. We enrolled 80, 78, and 74 patients who arrived at the hospital between 0-24, 24-48, and 48-72 hours after the first appearance of symptoms, respectively. DNA genotyping was done by the tetra-primer ARMS-PCR method. Circulating MIAT levels were evaluated by real-time polymerase chain reaction (PCR).

Results: The GT genotype of MIAT rs1894720 showed a significant association with the risk of IS (OR=3.53, 95% CI=2.13-5.84, P<0.001). MIAT expression was higher relative to the control within the first hours after IS. The MIAT levels in IS patients with rs1894720 (GT) were significantly lower relative to patients who had the GG and TT genotypes. Linear regression model indicated a significant correlation between MIAT expression with atherosclerotic risk factors and types of stroke in IS patients. Receiver operating characteristic (ROC) curve analysis showed that the level of lncRNA MIAT after IS could be diagnostic with an area under the curve (AUC) of 0.82. The sensitivity and specificity were 80.17 and 67.24%, respectively (P<0.001).

Conclusion: Our study demonstrated that the MIAT rs1894720 polymorphism (GT) might increase the risk of IS in the Iranian population. MIAT expression was up-regulated in our IS patients. Hence, it could be a diagnostic biomarker for IS.

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lncRNA MIAT rs1894720 基因多态性与缺血性脑卒中风险及缺血性脑卒中后血液中 lncRNA MIAT 表达水平的关系：一项病例对照研究。

目的：遗传因素在缺血性脑卒中（IS）的发生和发展中起着至关重要的作用。Rs1894720 多态性是长非编码 RNA（lncRNA）心肌梗死相关转录本（MIAT）位点的八个单核苷酸多态性（SNPs）之一。研究的目的是lncRNA MIAT rs1894720多态性通过减少lncRNA MIAT的表达来降低IS风险：在这项病例对照研究中，我们研究了 232 名伊朗患者和 232 名对照组。血样采集自出现中风症状后不同时间入院的患者。我们分别选取了首次出现症状后 0-24 小时、24-48 小时和 48-72 小时到达医院的 80、78 和 74 名患者。DNA 基因分型采用四引物 ARMS-PCR 方法。循环中的MIAT水平通过实时聚合酶链反应（PCR）进行评估：MIAT rs1894720的GT基因型与IS风险有显著相关性（OR=3.53，95% CI=2.13-5.84）。与 GG 和 TT 基因型患者相比，rs1894720（GT）型 IS 患者的 MIAT 水平明显较低。线性回归模型表明，MIAT的表达与动脉粥样硬化危险因素和IS患者的中风类型有明显的相关性。接收者操作特征曲线（ROC）分析表明，IS后lncRNA MIAT的水平具有诊断意义，其曲线下面积（AUC）为0.82。灵敏度和特异度分别为 80.17% 和 67.24%（结论：我们的研究表明，MIAT 基因变异位点可用于诊断 IS：我们的研究表明，MIAT rs1894720 多态性（GT）可能会增加伊朗人群罹患 IS 的风险。在我们的 IS 患者中，MIAT 表达上调。因此，它可以作为 IS 的诊断生物标志物。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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