Methyl jasmonate differentially and tissue-specifically regulated the expression of arginine catabolism–related genes and proteins in Agaricus bisporus mushrooms during storage

IF 2.4 3区生物学 Q3 GENETICS & HEREDITY Fungal Genetics and Biology Pub Date : 2024-01-09 DOI:10.1016/j.fgb.2024.103864

Hanyue Jiang , Huadong Wang , Xiuhong Wang , Yating Wang , Rui Song , Shuai Yuan , Zhenchuan Fan , Demei Meng

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Abstract

Methyl jasmonate (MeJA)-regulated postharvest quality retention of Agaricus bisporus fruiting bodies is associated with arginine catabolism. However, the mechanism of MeJA-regulated arginine catabolism in edible mushrooms is still unclear. This study aimed to investigate the regulatory modes of MeJA on the expression of arginine catabolism–related genes and proteins in intact and different tissues of A. bisporus mushrooms during storage. Results showed that exogenous MeJA treatment activated endogenous JA biosynthesis in A. bisporus mushrooms, and differentially and tissue-specifically regulated the expression of arginine catabolism–related genes (AbARG, AbODC, AbSPE-SDH, AbSPDS, AbSAMDC, and AbASL) and proteins (AbARG, AbSPE-SDH, AbASL, and AbASS). MeJA caused no significant change in AbASS expression but resulted in a dramatic increase in AbASS protein level. Neither the expression of the AbSAMS gene nor the AbSAMS protein was conspicuously altered upon MeJA treatment. Additionally, MeJA reduced the contents of arginine and ornithine and induced the accumulation of free putrescine and spermidine, which was closely correlated with MeJA-regulated arginine catabolism–related genes and proteins. Hence, the results suggested that the differential and tissue-specific regulation of arginine catabolism–related genes and proteins by MeJA contributed to their selective involvement in the postharvest continuing development and quality retention of button mushrooms.

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茉莉酸甲酯对双孢蘑菇贮藏过程中精氨酸代谢相关基因和蛋白质的表达具有不同的组织特异性调控作用

茉莉酸甲酯（MeJA）调节双孢蘑菇子实体采收后的品质保持与精氨酸分解代谢有关。然而，MeJA 调节食用菌精氨酸分解代谢的机制仍不清楚。本研究旨在探讨 MeJA 在双孢蘑菇贮藏过程中对其完整组织和不同组织中精氨酸代谢相关基因和蛋白质表达的调控模式。结果表明，外源 MeJA 可激活双孢蘑菇内源 JA 的生物合成，并对精氨酸代谢相关基因（AbARG、AbODC、AbSPE-SDH、AbSPDS、AbSAMDC 和 AbASL）和蛋白（AbARG、AbSPE-SDH、AbASL 和 AbASS）的表达进行差异化和组织特异性调控。MeJA 不会导致 AbASS 表达的明显变化，但会导致 AbASS 蛋白水平的急剧上升。经 MeJA 处理后，AbSAMS 基因和 AbSAMS 蛋白的表达均无明显变化。此外，MeJA还降低了精氨酸和鸟氨酸的含量，并诱导游离的腐胺和亚精胺的积累，这与MeJA调控的精氨酸分解代谢相关基因和蛋白密切相关。因此，研究结果表明，MeJA 对精氨酸分解代谢相关基因和蛋白质的差异化和组织特异性调控有助于它们选择性地参与金针菇采后的持续生长和品质保持。

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来源期刊

Fungal Genetics and Biology 生物-遗传学

CiteScore

6.20

自引率

3.30%

发文量

审稿时长

85 days

期刊介绍： Fungal Genetics and Biology, formerly known as Experimental Mycology, publishes experimental investigations of fungi and their traditional allies that relate structure and function to growth, reproduction, morphogenesis, and differentiation. This journal especially welcomes studies of gene organization and expression and of developmental processes at the cellular, subcellular, and molecular levels. The journal also includes suitable experimental inquiries into fungal cytology, biochemistry, physiology, genetics, and phylogeny. Fungal Genetics and Biology publishes basic research conducted by mycologists, cell biologists, biochemists, geneticists, and molecular biologists. Research Areas include: • Biochemistry • Cytology • Developmental biology • Evolutionary biology • Genetics • Molecular biology • Phylogeny • Physiology.