Helene Solberg, Christina Bartholdy , Lone Hummelshøj Landsy, Lars Ole Andresen
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引用次数: 0
Abstract
Stability of anti-drug antibodies (ADAs) is important as ADA-analysis should be reliable over time at different storage conditions. Stability of anti-insulin antibodies in serum samples was assessed after short-term storage at different temperatures and after long-term storage at −20 °C. Correlation between measurements was tested and acceptance criteria for incurred sample reanalysis were applied. ADAs were stable after 72 h at 22 °C, after 2 weeks at 4 °C, and after 6.3 years at −20 °C. The study confirms that ADAs in serum are stable for several years at −20 °C and suggests that investigation of short- and long-term stability of ADAs is not needed if samples are handled at standard laboratory-conditions.
抗药物抗体（ADA）的稳定性非常重要，因为在不同的储存条件下，抗药物抗体分析应该是可靠的。我们评估了血清样本在不同温度下短期储存和在-20 °C下长期储存后抗胰岛素抗体的稳定性。测试了测量结果之间的相关性，并采用了相关样本重新分析的验收标准。ADA 在 22 °C 下 72 小时后、4 °C 下 2 周后以及 -20 °C 下 6.3 年后均保持稳定。这项研究证实，血清中的 ADA 在-20 °C下可稳定数年，并表明如果在标准实验室条件下处理样品，则无需对 ADA 的短期和长期稳定性进行研究。
期刊介绍：
The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells.
In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.
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