Changes in the expression of mexB, mexY, and oprD in clinical Pseudomonas aeruginosa isolates.

IF 4.4 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2024-01-01 DOI:10.2183/pjab.100.006

Yoshimi Matsumoto, Seiji Yamasaki, Kouhei Hayama, Ryota Iino, Hiroyuki Noji, Akihito Yamaguchi, Kunihiko Nishino

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Abstract

Changes in expression levels of drug efflux pump genes, mexB and mexY, and porin gene oprD in Pseudomonas aeruginosa were investigated in this study. Fifty-five multidrug-resistant P. aeruginosa (MDRP) strains were compared with 26 drug-sensitive strains and 21 strains resistant to a single antibiotic. The effect of the efflux inhibitor Phe-Arg-β-naphthylamide on drug susceptibility was determined, and gene expression was quantified using real-time quantitative real-time reverse transcription polymerase chain reaction. In addition, the levels of metallo-β-lactamase (MBL) and 6'-N-aminoglycoside acetyltransferase [AAC(6')-Iae] were investigated. Efflux pump inhibitor treatment increased the sensitivity to ciprofloxacin, aztreonam, and imipenem in 71%, 73%, and 29% of MDRPs, respectively. MBL and AAC(6')-Iae were detected in 38 (69%) and 34 (62%) MDRP strains, respectively. Meanwhile, 76% of MDRP strains exhibited more than 8-fold higher mexY expression than the reference strain PAO1. Furthermore, 69% of MDRP strains expressed oprD at levels less than 0.01-fold of those in PAO1. These findings indicated that efflux pump inhibitors in combination with ciprofloxacin or aztreonam might aid in treating MDRP infections.

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临床铜绿假单胞菌分离物中 mexB、mexY 和 oprD 表达的变化。

本研究调查了铜绿假单胞菌中药物外排泵基因 mexB 和 mexY 以及孔蛋白基因 oprD 表达水平的变化。55 株多重耐药铜绿假单胞菌（MDRP）与 26 株对药物敏感的菌株和 21 株对单一抗生素耐药的菌株进行了比较。测定了外排抑制剂 Phe-Arg-β-naphthylamide 对药物敏感性的影响，并使用实时定量反转录聚合酶链反应对基因表达进行了量化。此外，还研究了金属-β-内酰胺酶（MBL）和 6'-N-氨基糖苷乙酰转移酶 [AAC(6')-Iae]的水平。外排泵抑制剂治疗分别提高了 71%、73% 和 29% 的 MDRP 对环丙沙星、阿曲南和亚胺培南的敏感性。在 38 株（69%）和 34 株（62%）MDRP 菌株中分别检测到 MBL 和 AAC(6')-Iae。同时，76% 的 MDRP 菌株的 mexY 表达量比参考菌株 PAO1 高 8 倍以上。此外，69% 的 MDRP 菌株表达的 oprD 水平低于 PAO1 的 0.01 倍。这些发现表明，外排泵抑制剂与环丙沙星或阿曲南合用可能有助于治疗 MDRP 感染。

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来源期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences 生物-综合性期刊

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Proceedings of the Japan Academy Ser. B (PJA-B) is a scientific publication of the Japan Academy with a 90-year history, and covers all branches of natural sciences, except for mathematics, which is covered by the PJA-A. It is published ten times a year and is distributed widely throughout the world and can be read and obtained free of charge through the world wide web.