TENT5A Increases Glioma Malignancy and Promotes its Progression.

Recent patents on anti-cancer drug discovery Pub Date : 2024-01-09 DOI:10.2174/0115748928280901231206102637

Jiali Hu, Lei Zeng, Ronghuan Hu, Dan Gong, Mengmeng Liu, Jianwu Ding

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Abstract

Background: Recent studies reported that terminal nucleotidyltransferase 5A (TENT5A) is highly expressed in glioblastoma and associated with poor prognosis. In this work, we aim to specify the expression level of TENT5A in different grades of glioma and explore its role in glioma progression.

Methods: GEPIA online tools were used to perform the bioinformatic analysis. qRT-PCR, Western blot, and Immunohistochemistry were performed in glioma cells or tissues. Furthermore, CCK8, colony formation, transwell, flow cytometry and scratch assays were performed.

Results: TENT5A was highly expressed in glioma and its level was associated with the pathological grade of glioma. Knockdown of TENT5A suppressed cell proliferation, colony formation ability, cell invasion and migration. Overexpression of TENT5A was lethal to the glioma cells.

Conclusion: Our data showed that the expression of TENT5A is associated with the pathological grade of glioma. Knockdown of TENT5A decreased the ability of proliferation, invasion and migration of glioma cells. High levels of TENT5A in glioma cells are lethal. Therefore, TENT5A could be a new target for glioma treatment.

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TENT5A 增加胶质瘤的恶性程度并促进其进展

背景：最近有研究报道，末端核苷酸转移酶5A（TENT5A）在胶质母细胞瘤中高表达，并与不良预后相关。本研究旨在明确 TENT5A 在不同级别胶质瘤中的表达水平，并探讨其在胶质瘤进展中的作用：方法：使用 GEPIA 在线工具进行生物信息学分析，对胶质瘤细胞或组织进行 qRT-PCR、Western 印迹和免疫组化。此外，还进行了 CCK8、集落形成、transwell、流式细胞术和划痕试验：结果：TENT5A在胶质瘤中高表达，其水平与胶质瘤的病理分级有关。敲除 TENT5A 可抑制细胞增殖、集落形成能力、细胞侵袭和迁移。结论：我们的数据表明，TENT5A的表达与胶质瘤的病理分级有关。敲除 TENT5A 能降低胶质瘤细胞的增殖、侵袭和迁移能力。胶质瘤细胞中高水平的 TENT5A 具有致死性。因此，TENT5A可能是治疗胶质瘤的新靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Recent patents on anti-cancer drug discovery

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