Bovine viral diarrhoea viruses from New Zealand belong predominantly to the BVDV-1a genotype.

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES New Zealand veterinary journal Pub Date : 2024-03-01 Epub Date: 2024-02-04 DOI:10.1080/00480169.2023.2291039

M Dunowska, R Lal, S D Dissanayake, S D Bond, E Burrows, J Moffat, L Howe

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Abstract

Aim: To determine which genotypes of bovine viral diarrhoea virus (BVDV) circulate among cattle in New Zealand.

Methods: Samples comprised BVDV-1-positive sera sourced from submissions to veterinary diagnostic laboratories in 2019 (n = 25), 2020 (n = 59) and 2022 (n = 74) from both beef and dairy herds, as well as archival BVDV-1 isolates (n = 5). Fragments of the 5' untranslated region (5' UTR) and glycoprotein E2 coding sequence of the BVDV genome were amplified and sequenced. The sequences were aligned to each other and to international BVDV-1 sequences to determine their similarities and phylogenetic relationships. The 5' UTR sequences were also used to create genetic haplotype networks to determine if they were correlated with selected traits (location, type of farm, and year of collection).

Results: The 5' UTR sequences from New Zealand BVDV were closely related to each other, with pairwise identities between 89% and 100%. All clustered together and were designated as BVDV-1a (n = 144) or BVDV-1c (n = 5). There was no evidence of a correlation between the 5' UTR sequence and the geographical origin within the country, year of collection or the type of farm. Partial E2 sequences from New Zealand BVDV (n = 76) showed 74-100% identity to each other and clustered in two main groups. The subtype assignment based on the E2 sequence was the same as based on the 5' UTR analysis. This is the first comprehensive analysis of genomic variability of contemporary New Zealand BVDV based on the analysis of the non-coding (5' UTR) and coding (E2) sequences.

Conclusions and clinical relevance: Knowledge of the diversity of the viruses circulating in the country is a prerequisite for the development of effective control strategies, including a selection of suitable vaccines. The data presented suggest that New Zealand BVDV are relatively homogeneous, which should facilitate eradication efforts including selection or development of the most suitable vaccines.

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新西兰的牛病毒性腹泻病毒主要属于 BVDV-1a 基因型。

目的：确定新西兰牛只中流行的牛病毒性腹泻病毒（BVDV）基因型：样本包括 2019 年（n = 25）、2020 年（n = 59）和 2022 年（n = 74）提交给兽医诊断实验室的 BVDV-1 阳性血清（来自肉牛和奶牛群），以及存档的 BVDV-1 分离物（n = 5）。对BVDV基因组的5' 非翻译区（5' UTR）和糖蛋白E2编码序列片段进行了扩增和测序。这些序列相互比对，并与国际 BVDV-1 序列比对，以确定它们的相似性和系统发育关系。5' UTR序列还被用于创建遗传单倍型网络，以确定它们是否与所选性状（地点、农场类型和采集年份）相关：结果：新西兰 BVDV 的 5' UTR 序列彼此密切相关，配对相同度介于 89% 和 100% 之间。所有序列都聚集在一起，被命名为 BVDV-1a（n = 144）或 BVDV-1c（n = 5）。没有证据表明 5' UTR 序列与国内的地理来源、采集年份或农场类型有关。新西兰 BVDV 的部分 E2 序列（n = 76）显示出 74-100% 的相同性，并分为两大类。根据 E2 序列划分的亚型与根据 5' UTR 分析划分的亚型相同。这是首次基于非编码（5' UTR）和编码（E2）序列分析对当代新西兰BVDV基因组变异性进行的全面分析：了解该国流行病毒的多样性是制定有效控制策略（包括选择合适疫苗）的先决条件。所提供的数据表明，新西兰的 BVDV 相对比较单一，这应有助于根除工作，包括选择或开发最合适的疫苗。

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来源期刊

New Zealand veterinary journal 农林科学-兽医学

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

12-24 weeks

期刊介绍： The New Zealand Veterinary Journal (NZVJ) is an international journal publishing high quality peer-reviewed articles covering all aspects of veterinary science, including clinical practice, animal welfare and animal health. The NZVJ publishes original research findings, clinical communications (including novel case reports and case series), rapid communications, correspondence and review articles, originating from New Zealand and internationally. Topics should be relevant to, but not limited to, New Zealand veterinary and animal science communities, and include the disciplines of infectious disease, medicine, surgery and the health, management and welfare of production and companion animals, horses and New Zealand wildlife. All submissions are expected to meet the highest ethical and welfare standards, as detailed in the Journal’s instructions for authors.