Novel vaccine candidates of Bordetella pertussis identified by reverse vaccinology

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-01-12 DOI:10.1016/j.biologicals.2023.101740

Gloria Paulina Monterrubio-López, José Luis Llamas-Monroy, Ángel Antonio Martínez-Gómez, Karen Delgadillo-Gutiérrez

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Abstract

Whooping cough is a disease caused by Bordetella pertussis, whose morbidity has increased, motivating the improvement of current vaccines. Reverse vaccinology is a strategy that helps identify proteins with good characteristics fast and with fewer resources. In this work, we applied reverse vaccinology to study the B. pertussis proteome and pangenome with several in-silico tools. We analyzed the B. pertussis Tohama I proteome with NERVE software and compared 234 proteins with B. parapertussis, B. bronchiseptica, and B. holmessi. VaxiJen was used to calculate an antigenicity value; our threshold was 0.6, selecting 84 proteins. The candidates were depurated and grouped in eight family proteins to select representative candidates, according to bibliographic information and their immunological response predicted with ABCpred, Bcepred, IgPred, and C-ImmSim. Additionally, a pangenome study was conducted with 603 B. pertussis strains and PanRV software, identifying 3421 core proteins that were analyzed to select the best candidates. Finally, we selected 15 proteins from the proteome study and seven proteins from the pangenome analysis as good vaccine candidates.

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通过反向疫苗学鉴定百日咳博德特氏菌新型候选疫苗

百日咳是由百日咳杆菌（Bordetella pertussis）引起的一种疾病，其发病率不断上升，促使人们对现有疫苗进行改进。反向疫苗学是一种有助于以更少的资源快速识别具有良好特性的蛋白质的策略。在这项工作中，我们利用反向疫苗学研究了百日咳杆菌蛋白质组和泛基因组。我们利用 NERVE 软件分析了百日咳杆菌 Tohama I 蛋白质组，并将 234 个蛋白质与副百日咳杆菌、支气管败血病杆菌和霍乱弧菌进行了比较。VaxiJen 用于计算抗原性值；我们的阈值为 0.6，选出了 84 个蛋白质。根据文献信息和用 ABCpred、Bcepred、IgPred 和 C-ImmSim 预测的免疫反应，对候选蛋白进行了去蛋白化处理，并按八个家族蛋白分组，以选出具有代表性的候选蛋白。此外，我们还利用 603 株百日咳杆菌菌株和 PanRV 软件进行了泛基因组研究，确定了 3421 个核心蛋白，并对这些蛋白进行了分析，以选出最佳候选蛋白。最后，我们从蛋白质组研究中选出了 15 个蛋白质，并从庞基因组分析中选出了 7 个蛋白质作为候选疫苗。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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