Biofabrication of silver nanoparticles using diallyl sulphide (DAS) and comparison of cytotoxic potential of DAS-AgNPs and Kodaikanal Hill garlic in human cervical cancer

Zeitschrift für Physikalische Chemie Pub Date : 2024-01-10 DOI:10.1515/zpch-2023-0454

Pranjal Priya, Uma Maheswari Nallal, Muthuswamy Razia, S. Sivaramakrishnan, Kannan Prabha, Lakshminarayanan Srimathi Priya, Suresh Kumar Kabeen, Karuppaiya Durga, Manikandan Ayyar

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Abstract

Abstract Garlic (Allium sativum) exhibit well defined therapeutic properties due to presence of distinct bioactive compounds, specifically organosulphur compounds. Spectral analysis by UV–Vis and FT-IR confirmed interaction of different functional groups and free electrons with the electromagnetic waves showed presence of different bioactive compounds formulated silver nanoparticles (AgNPs) using diallyl sulphide (DAS) also done for aqueous garlic extract. Different assays performed for confirming the presence of antibacterial and anticancer properties of DAS-AgNPs and garlic extract. The minimum inhibitory concentration required to inhibit Gram negative (Escherichia coli, V. cholera) and Gram positive (S. aureus, B. cereus) were found lesser in DAS-AgNPs followed by garlic extract. Cytotoxicity effect confirmed by DAS-AgNPs and A. sativum towards HeLa cell line by MTT assay. The IC50 values obtained for MTT assay were at 24.09 μg/ml in DAS-AgNPs and 129.22 μg/ml in garlic extract. Anticancer efficacy was found more potent in DAS-AgNPs as compared with aqueous garlic extract.

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利用二烯丙基硫醚（DAS）进行银纳米粒子的生物制造，并比较 DAS-AgNPs 和 Kodaikanal Hill 大蒜对人类宫颈癌的细胞毒性潜力

摘要大蒜（Allium sativum）因含有独特的生物活性化合物，特别是有机硫化合物，而具有明确的治疗特性。紫外-可见光和傅立叶变换红外光谱分析证实了不同官能团和自由电子与电磁波的相互作用，显示了使用二烯丙基硫醚（DAS）配制银纳米粒子（AgNPs）的不同生物活性化合物的存在，大蒜水提取物也是如此。为证实 DAS-AgNPs 和大蒜提取物的抗菌和抗癌特性，进行了不同的检测。发现 DAS-AgNPs 抑制革兰氏阴性菌（大肠杆菌、霍乱弧菌）和革兰氏阳性菌（金黄色葡萄球菌、蜡样芽孢杆菌）所需的最小抑菌浓度低于大蒜提取物。通过 MTT 试验证实了 DAS-AgNPs 和 A. sativum 对 HeLa 细胞系的细胞毒性作用。在 MTT 试验中，DAS-AgNPs 的 IC50 值为 24.09 μg/ml，大蒜提取物的 IC50 值为 129.22 μg/ml。与大蒜水提取物相比，DAS-AgNPs 的抗癌效果更强。

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Zeitschrift für Physikalische Chemie

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