The influence of simulated weightlessness on the composition and function of gut microbiota and bile acid metabolism products

IF 2.9 3区生物学 Q2 ASTRONOMY & ASTROPHYSICS Life Sciences in Space Research Pub Date : 2024-01-14 DOI:10.1016/j.lssr.2024.01.002

Min Wang , Sheng Chen , Changqing Zhong , Lei Liu , Guodong Wang , Xin Huang , Xiaoman Yang , Heming Yang , Lianyong Li

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Abstract

The aim of this study was to investigate the effects of simulated weightlessness on gut microbiota, bile acid metabolism, and inflammatory cytokines compared to the control group.

The study compared the changes in gut microbiota at the phylum and genus levels in the feces of control and weightlessness rats after 1 and 8 weeks using fecal 16S rRNA sequencing. In the weightlessness group, there was an increase in the proportion of anaerobic bacteria and biofilm-forming bacteria, and a decrease in the proportion of aerobic and Gram-negative bacteria. Further investigations explored the impact of weightlessness on bile acid metabolism products.

The levels of glycine ursodeoxycholic acid, glycine chenodeoxycholic acid, glycine deoxycholic acid and glycine cholic acid levels were lower in rats undergoing weightlessness for 1 week compared to the control group.Moreover, the study examined the relationship between gut microbiota and bile acid metabolism products.It was observed that, unlike the control group, there were significant positive correlations between Planctomycetes, Proteobacteria, Synergistetes, and GUDCA levels in rats after 1 week of weightlessness. Finally, ELISA results indicated significant differences in the levels of MDA, GSH, NLRP3, and SIgA inflammatory cytokines between rats undergoing weightlessness for 1 week and the control group rats.

Our research confirmed that the simulated weightlessness environment significantly affects the gut microbiota and bile acid metabolism in rats, potentially leading to changes in inflammatory cytokines and causing intestinal tissue inflammation. Further exploring the relationship between gut microbiota and bile acid metabolism under weightless conditions will be crucial for understanding the functional changes in the intestines caused by weightlessness.

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模拟失重对肠道微生物群和胆汁酸代谢产物的组成和功能的影响

这项研究的目的是，与对照组相比，调查模拟失重对肠道微生物群、胆汁酸代谢和炎症细胞因子的影响。研究采用粪便16S rRNA测序法，比较了对照组和失重组大鼠1周和8周后粪便中肠道微生物群在门和属水平上的变化。在失重组中，厌氧菌和生物膜形成菌的比例有所增加，而需氧菌和革兰氏阴性菌的比例有所下降。与对照组相比，失重 1 周的大鼠体内甘氨酸熊去氧胆酸、甘氨酸辰去氧胆酸、甘氨酸去氧胆酸和甘氨酸胆酸的水平较低。与对照组不同，失重 1 周后大鼠的 Planctomycetes、Proteobacteria、Synergistetes 和 GUDCA 水平之间存在显著的正相关。最后，ELISA 结果表明，失重 1 周的大鼠与对照组大鼠的 MDA、GSH、NLRP3 和 SIgA 炎症细胞因子水平存在显著差异。我们的研究证实，模拟失重环境会显著影响大鼠的肠道微生物群和胆汁酸代谢，可能导致炎症细胞因子的变化，引起肠道组织炎症。进一步探索失重条件下肠道微生物群与胆汁酸代谢之间的关系，对于了解失重导致的肠道功能变化至关重要。

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来源期刊

Life Sciences in Space Research Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

5.30

自引率

8.00%

发文量

期刊介绍： Life Sciences in Space Research publishes high quality original research and review articles in areas previously covered by the Life Sciences section of COSPAR''s other society journal Advances in Space Research. Life Sciences in Space Research features an editorial team of top scientists in the space radiation field and guarantees a fast turnaround time from submission to editorial decision.