Immunohistochemical expression of anaplastic lymphoma kinase in neuroblastoma and its relations with some clinical and histopathological features.

IF 1.7 Q3 PATHOLOGY Journal of Pathology and Translational Medicine Pub Date : 2024-01-01 Epub Date: 2024-01-10 DOI:10.4132/jptm.2023.12.07
Thu Dang Anh Phan, Thao Quyen Nguyen, Nhi Thuy To, Thien Ly Thanh, Dat Quoc Ngo
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Abstract

Background: Anaplastic lymphoma kinase (ALK) mutations have been identified as a prominent cause of some familial and sporadic neuroblastoma (NB). ALK expression in NB and its relationship with clinical and histopathological features remains controversial. This study investigated ALK expression and its potential relations with these features in NB.

Methods: Ninety cases of NB at the Department of Pathology, University of Medicine and Pharmacy at Ho Chi Minh City, Viet Nam from 01/01/2018 to 12/31/2021, were immunohistochemically stained with ALK (D5F3) antibody. The ALK expression and its relations with some clinical and histopathological features were investigated.

Results: The rate of ALK expression in NB was 91.1%. High ALK expression (over 50% of tumor cells were positive with moderate-strong intensity) accounted for 65.6%, and low ALK expression accounted for 34.4%. All the MYCN-amplified NB patients had ALK immunohistochemistry positivity, most cases had high ALK protein expression. The undifferentiated subtype of NB had a lower ALK-positive rate than the poorly differentiated and differentiated subtype. The percentages of ALK positivity were significantly higher in more differentiated histological types of NB (p = .024). There was no relation between ALK expression and: age group, sex, primary tumor location, tumor stage, MYCN status, clinical risk, Mitotic-Karyorrhectic Index, prognostic group, necrosis, and calcification.

Conclusions: ALK was highly expressed in NB. ALK expression was not related to several clinical and histopathological features. More studies are needed to elucidate the association between ALK expression and ALK gene status and to investigate disease progression, especially the oncogenesis of ALK-positive NB.

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神经母细胞瘤中无性淋巴瘤激酶的免疫组化表达及其与一些临床和组织病理学特征的关系
背景:无细胞淋巴瘤激酶(ALK)突变已被确定为一些家族性和散发性神经母细胞瘤(NB)的主要病因。ALK在NB中的表达及其与临床和组织病理学特征的关系仍存在争议。本研究调查了ALK在NB中的表达及其与这些特征的潜在关系:方法:用ALK(D5F3)抗体对越南胡志明市医药大学病理系2018年1月1日至2021年12月31日的90例NB病例进行免疫组化染色。研究了ALK的表达及其与一些临床和组织病理学特征的关系:结果:ALK在NB中的表达率为91.1%。结果:ALK在NB中的表达率为91.1%,ALK高表达(超过50%的肿瘤细胞呈中强阳性)占65.6%,ALK低表达占34.4%。所有MYCN扩增的NB患者均有ALK免疫组化阳性,大多数病例有ALK蛋白高表达。未分化亚型NB的ALK阳性率低于分化差和分化亚型。在分化程度较高的组织学类型的NB中,ALK阳性率明显更高(p = .024)。ALK表达与年龄组、性别、原发肿瘤位置、肿瘤分期、MYCN状态、临床风险、有丝分裂-核分裂指数、预后组、坏死和钙化之间没有关系:结论:ALK在NB中高表达。结论:ALK在NB中高表达,ALK的表达与一些临床和组织病理学特征无关。需要开展更多研究,以阐明ALK表达与ALK基因状态之间的关联,并研究疾病进展,尤其是ALK阳性NB的肿瘤发生。
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来源期刊
CiteScore
5.00
自引率
4.20%
发文量
45
审稿时长
14 weeks
期刊介绍: The Journal of Pathology and Translational Medicine is an open venue for the rapid publication of major achievements in various fields of pathology, cytopathology, and biomedical and translational research. The Journal aims to share new insights into the molecular and cellular mechanisms of human diseases and to report major advances in both experimental and clinical medicine, with a particular emphasis on translational research. The investigations of human cells and tissues using high-dimensional biology techniques such as genomics and proteomics will be given a high priority. Articles on stem cell biology are also welcome. The categories of manuscript include original articles, review and perspective articles, case studies, brief case reports, and letters to the editor.
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