Design and development of a portable low-cost QCM-based system for liquid biosensing.

IF 3 4区医学 Q3 ENGINEERING, BIOMEDICAL Biomedical Microdevices Pub Date : 2024-01-18 DOI:10.1007/s10544-024-00696-0

Mohamed Adel, Ahmed Allam, Ashraf E Sayour, Hani F Ragai, Shinjiro Umezu, Ahmed M R Fath El-Bab

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Abstract

Quartz crystal microbalance (QCM) is a versatile sensing platform that has gained increasing attention for its use in bioapplications due to its high sensitivity, real-time measurement capabilities, and label-free detection. This article presents a portable QCM system for liquid biosensing that uses a modified Hartley oscillator to drive 14 mm-diameter commercial QCM sensors. The system is designed to be low-cost, easy to use, and highly sensitive, making it ideal for various bioapplications. A new flow cell design to deliver samples to the surface of the sensor has been designed, fabricated, and tested. For portability and miniaturization purposes, a micropump-based pumping system is used in the current system. The system has a built-in temperature controller allowing for accurate frequency measurements. In addition, the system can be used in benchtop mode. The capability of the present system to be used in liquid biosensing is demonstrated through an experimental test for sensitivity to changes in the viscosity of glycerol samples. It was found to have a sensitivity of 263.51 Hz/mPa.s using a 10 MHz QCM sensor. Future work regarding potential applications was suggested.

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设计和开发基于 QCM 的便携式低成本液体生物传感系统。

石英晶体微天平（QCM）是一种多功能传感平台，因其灵敏度高、实时测量能力强和免标记检测等特点，在生物应用中的应用日益受到关注。本文介绍了一种用于液体生物传感的便携式 QCM 系统，该系统使用改进的 Hartley 振荡器来驱动 14 毫米直径的商用 QCM 传感器。该系统设计成本低、使用方便、灵敏度高，是各种生物应用的理想之选。我们设计、制造并测试了一种新的流动池设计，用于将样品输送到传感器表面。为了便于携带和小型化，目前的系统采用了基于微型泵的泵送系统。该系统内置温度控制器，可进行精确的频率测量。此外，该系统还可在台式模式下使用。通过对甘油样品粘度变化灵敏度的实验测试，证明了本系统在液体生物传感中的应用能力。通过使用 10 MHz QCM 传感器，发现其灵敏度为 263.51 Hz/mPa.s。建议今后开展有关潜在应用的工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.