Identification of the Csr global regulatory system mediated by small RNA decay in Aeromonas salmonicida.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2024-07-20 Epub Date: 2024-01-18 DOI:10.2323/jgam.2023.12.004
Olga Gladyshchuk, Masaki Yoshida, Koume Togashi, Hayuki Sugimoto, Kazushi Suzuki
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Abstract

We investigated the presence and functionality of the carbon storage regulator (Csr) system in Aeromonas salmonicida SWSY-1.411. CsrA, an RNA-binding protein, shared 89% amino acid sequence identity with Escherichia coli CsrA. CsrB/C sRNAs exhibited a typical stem-loop structure, with more GGA motifs, which bind CsrA, than E. coli. CsrD had limited sequence identity with E. coli CsrD; however, it contained the conserved GGDEF and EAL domains. Functional analysis in E. coli demonstrated that the Csr system of A. salmonicida influences glycogen biosynthesis, biofilm formation, motility, and stability of both CsrB and CsrC sRNAs. These findings suggest that in A. salmonicida, the Csr system affects phenotypes like its E. coli counterpart. In A. salmonicida, defects in csr homologs affected biofilm formation, motility, and chitinase production. However, glycogen accumulation and protease production were unaffected. The expression of flagellar-related genes and chitinase genes was suppressed in the csrA-deficient A. salmonicida. Northern blot analysis indicated the stabilization of CsrB and CsrC in the csrD-deficient A. salmonicida. Similar to that in E. coli, the Csr system in A. salmonicida comprises the RNA-binding protein CsrA, the sRNAs CsrB and CsrC, and the sRNA decay factor CsrD. This study underscores the conservation and functionality of the Csr system and raises questions about its regulatory targets and mechanisms in A. salmonicida.

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鉴定沙门氏菌中由小 RNA 降解介导的 Csr 全局调控系统。
我们研究了沙门氏菌 SWSY-1.411 中碳储存调节器(Csr)系统的存在和功能。CsrA是一种RNA结合蛋白,与大肠杆菌CsrA有89%的氨基酸序列相同性。CsrB/C sRNA 具有典型的茎环结构,与大肠杆菌相比,具有更多与 CsrA 结合的 GGA 基序。CsrD 与大肠杆菌 CsrD 的序列同一性有限;但是,它含有保守的 GGDEF 和 EAL 结构域。在大肠杆菌中进行的功能分析表明,沙门氏菌的 Csr 系统影响糖原生物合成、生物膜形成、运动以及 CsrB 和 CsrC sRNA 的稳定性。这些研究结果表明,在沙门氏菌中,Csr 系统对表型的影响与大肠杆菌类似。在沙门氏菌中,csr 同源物的缺陷会影响生物膜的形成、运动性和几丁质酶的产生。然而,糖原积累和蛋白酶的产生则不受影响。在 csrA 缺陷的鲑鱼中,鞭毛相关基因和几丁质酶基因的表达受到抑制。Northern 印迹分析表明,在 csrD 缺失的沙门氏菌中,CsrB 和 CsrC 稳定。与大肠杆菌中的Csr系统相似,沙门氏菌中的Csr系统包括RNA结合蛋白CsrA、sRNA CsrB和CsrC以及sRNA衰变因子CsrD。这项研究强调了 Csr 系统的保守性和功能性,并提出了有关沙门氏菌中 Csr 系统调控目标和机制的问题。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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