Molecular Characteristics of Staphylococcus aureus Isolates form Food-Poisoning Outbreaks (2011-2022) in Sichuan, China.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Foodborne pathogens and disease Pub Date : 2024-05-01 Epub Date: 2024-01-19 DOI:10.1089/fpd.2023.0097

Jie Zheng, Li Liu, Guo Chen, Wenping Xu, Yulan Huang, Gaopeng Lei, Weifeng Huang, Hong Lv, Xiaorong Yang

{"title":"Molecular Characteristics of Staphylococcus aureus Isolates form Food-Poisoning Outbreaks (2011-2022) in Sichuan, China.","authors":"Jie Zheng, Li Liu, Guo Chen, Wenping Xu, Yulan Huang, Gaopeng Lei, Weifeng Huang, Hong Lv, Xiaorong Yang","doi":"10.1089/fpd.2023.0097","DOIUrl":null,"url":null,"abstract":"Staphylococcal food poisoning (SFP) is one of the most common foodborne diseases in the world. This study aimed to investigate the molecular epidemiological characteristics of Staphylococcus aureus isolated from SFP. A total of 103 S. aureus isolates were obtained during 2011-2022 in Sichuan, southwest China. All isolates were tested for the genomic characteristics and phylogenetic analysis by performing whole-genome sequencing. Multilocus sequence typing analysis showed 17 multilocus sequence types (STs), ST7 (23.30%), ST5 (22.33%), and ST6 (16.50%) being the most common. A total of 45 virulence genes were detected, 22 of which were staphylococcal enterotoxin (SE) genes. Among the identified SE genes, selX exhibited the highest prevalence (86.4%). All isolates carried at least one SE gene. The results of the antimicrobial resistance (AMR) gene detection revealed 41 AMR genes of 12 classes. β-lactam resistance genes (blal, blaR1, blaZ) and tetracycline resistance gene (tet(38)) exhibited a higher prevalence rate. Core genome single nucleotide polymorphism showed phylogenetic clustering of the isolates with the same region, year, and ST. The results indicated that the SFP isolates in southwest of China harbored multiple toxin and resistance genes, with a high prevalence of new SEs. Therefore, it is important to monitor the antimicrobial susceptibility and SE of S. aureus to reduce the potential risks to public health.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"323-330"},"PeriodicalIF":1.9000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/fpd.2023.0097","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/19 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Staphylococcal food poisoning (SFP) is one of the most common foodborne diseases in the world. This study aimed to investigate the molecular epidemiological characteristics of Staphylococcus aureus isolated from SFP. A total of 103 S. aureus isolates were obtained during 2011-2022 in Sichuan, southwest China. All isolates were tested for the genomic characteristics and phylogenetic analysis by performing whole-genome sequencing. Multilocus sequence typing analysis showed 17 multilocus sequence types (STs), ST7 (23.30%), ST5 (22.33%), and ST6 (16.50%) being the most common. A total of 45 virulence genes were detected, 22 of which were staphylococcal enterotoxin (SE) genes. Among the identified SE genes, selX exhibited the highest prevalence (86.4%). All isolates carried at least one SE gene. The results of the antimicrobial resistance (AMR) gene detection revealed 41 AMR genes of 12 classes. β-lactam resistance genes (blal, blaR1, blaZ) and tetracycline resistance gene (tet(38)) exhibited a higher prevalence rate. Core genome single nucleotide polymorphism showed phylogenetic clustering of the isolates with the same region, year, and ST. The results indicated that the SFP isolates in southwest of China harbored multiple toxin and resistance genes, with a high prevalence of new SEs. Therefore, it is important to monitor the antimicrobial susceptibility and SE of S. aureus to reduce the potential risks to public health.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

中国四川食物中毒疫情（2011-2022 年）中金黄色葡萄球菌分离物的分子特征。

葡萄球菌食物中毒（SFP）是世界上最常见的食源性疾病之一。本研究旨在调查从食物中毒中分离出的金黄色葡萄球菌的分子流行病学特征。研究人员于 2011-2022 年间在中国西南部的四川省共获得 103 个金黄色葡萄球菌分离株。通过全基因组测序，对所有分离株进行了基因组特征检测和系统发育分析。多焦点序列分型分析表明，分离物共有17种多焦点序列类型（ST），其中以ST7（23.30%）、ST5（22.33%）和ST6（16.50%）最为常见。共检测到 45 个毒力基因，其中 22 个是葡萄球菌肠毒素（SE）基因。在已发现的 SE 基因中，selX 的流行率最高（86.4%）。所有分离株都携带至少一种 SE 基因。抗菌药耐药性（AMR）基因检测结果显示有 12 类 41 个 AMR 基因。β-内酰胺耐药基因（blal、blaR1、blaZ）和四环素耐药基因（tet(38)）的流行率较高。核心基因组单核苷酸多态性显示，具有相同地区、年份和 ST 的分离物在系统发育上呈聚类。结果表明，中国西南地区的 SFP 分离物携带多种毒素基因和抗性基因，新 SE 的流行率较高。因此，监测金黄色葡萄球菌的抗菌药敏感性和耐药性对降低公共卫生的潜在风险非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.