Effective production of β-sitosteryl oleate using a highly thermal-tolerant immobilized lipase in a solvent-free system

IF 1.9 4区 农林科学 Q3 CHEMISTRY, APPLIED Journal of the American Oil Chemists Society Pub Date : 2024-01-17 DOI:10.1002/aocs.12813
Kexin Chen, Xuan Liu, Bo Ouyang, Dongming Lan, Yonghua Wang
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Abstract

β-Sitosteryl oleate, renowned for its diverse beneficial bioactivities, holds significant promise as a potential ingredient in functional foods. This study reports the superior performance of β-sitosteryl oleate facilitated by lipase UM1 (lipase from marine Streptomyces sp. W007, immobilized on XAD1180 resin) as a biocatalyst in a solvent-free system, in comparison to commercial enzymes Novozym 435 (lipase B from Candida antarctica, immobilized on a macroporous acrylic resin), Lipozyme TL IM (lipase from Thermomyces lanuginosus, immobilized on a non-compressible silica gel carrier), and Lipozyme RM IM (lipase from Rhizomucor miehei, immobilized on a macroporous acrylic resin). Remarkably, an over 98% yield was achieved under the optimal conditions: a substrate molar ratio of β-sitosterol to oleic acid of 1:4, lipase loading of 150 U, and a reaction temperature of 60°C. The process exhibited substantial resilience and effectiveness, maintaining a degree of esterification above 95% even after five recycles. Following this, the synthesis was successfully scaled up by 100-fold, with the product isolated through molecular distillation and confirmed using ultra-performance liquid chromatography mass spectrometry (UPLC-MS) and Fourier transform infrared spectroscopy (FT-IR) analytical techniques. These results underscore lipase UM1 as a promising catalyst for the industrial-scale synthesis of β-sitosteryl oleate, fostering expanded avenues for its utilization in the functional food industry.

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在无溶剂体系中使用高耐热性固定化脂肪酶有效生产 β-谷甾醇油酸酯
β-谷甾醇油酸酯以其多种有益的生物活性而闻名,有望成为功能食品的潜在成分。本研究报告了脂肪酶 UM1(来自海洋链霉菌 W007 的脂肪酶,固定在 XAD1180 树脂上)促进 β-谷甾醇油酸酯的卓越性能。W007,固定在 XAD1180 树脂上)作为无溶剂体系中的生物催化剂,与商业酶 Novozym 435(来自南极念珠菌的脂肪酶 B,固定在大孔丙烯酸树脂上)相比,具有更优越的性能、Lipozyme TL IM(固定在非压缩硅胶载体上的热酵母菌脂肪酶)和 Lipozyme RM IM(固定在大孔丙烯酸树脂上的根瘤菌脂肪酶)。值得注意的是,在β-谷甾醇与油酸的底物摩尔比为 1:4、脂肪酶负载量为 150 U、反应温度为 60°C 的最佳条件下,产率超过 98%。该工艺表现出很强的适应性和有效性,即使经过五次循环,酯化程度仍保持在 95% 以上。随后,合成过程成功地扩大了 100 倍,产物通过分子蒸馏分离出来,并使用超高效液相色谱-质谱(UPLC-MS)和傅立叶变换红外光谱(FT-IR)分析技术进行了确认。这些结果表明,脂肪酶 UM1 是一种很有前景的催化剂,可用于工业规模的 β-谷甾醇油酸酯合成,为其在功能食品工业中的应用开辟了更广阔的途径。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
95
审稿时长
2.4 months
期刊介绍: The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of year­to­year, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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Issue Information Issue Information JAOCS special issue on advancement in plant protein-based emulsions Issue Information Issue Information
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