Amphipterygium adstringens (cuachalalate) extract by supercritical CO2

Abstract

This study delineates the empirical findings derived from the extraction of Sarsasapogenin from Amphipterygium Adstringens, commonly known as Cuachalalate, utilizing supercritical carbon dioxide (CO₂) as a solvent. The experiments were conducted within a temperature spectrum of 314 to 328.4 K and a pressure range of 8–22 MPa. The operational duration was set at 90 min with a CO₂ solvent flow rate oscillating between 40 and 50 mL/min. The equipment employed was predicated on the dynamic-analytical method. The extract´s qualitative determination was performed using a liquid chromatograph interfaced with a mass spectrometer (HPLC-MS). In contrast, the quantitative analysis was executed employing Ultraviolet Visible spectroscopy (UV–Vis) and Fourier Transform Infrared Spectroscopy (FTIR). Sarsasapogenin was identified as the representative compound in the Cuachalalate bark extract. The quantitative evaluations revealed that the maximum extract yield, amounting to 26.56 mg, was obtained under optimal operating conditions. Furthermore, this study investigated the influence of pressure and temperature variations on the extract´s yield.