Preparation, Characterization, and Anticancer Activity Assessment of Chitosan/TPP Nanoparticles Loaded with Echis carinatus Venom.

IF 2.6 4区医学 Q3 CHEMISTRY, MEDICINAL Anti-cancer agents in medicinal chemistry Pub Date : 2024-01-01 DOI:10.2174/0118715206279731231129105221

Maral Mahboubi Kancha, Mohsen Mehrabi, Fatemeh Sadat Bitaraf, Hamid Vahedi, Morteza Alizadeh, Andreas Bernkop-Schnürch

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Abstract

Aims and background: Echis carinatus venom is a toxic substance naturally produced by special glands in this snake species. Alongside various toxic properties, this venom has been used for its therapeutic effects, which are applicable in treating various cancers (liver, breast, etc.).

Objective: Nanotechnology-based drug delivery systems are suitable for protecting Echis carinatus venom against destruction and unwanted absorption. They can manage its controlled transfer and absorption, significantly reducing side effects.

Methods: In the present study, chitosan nanoparticles were prepared using the ionotropic gelation method with emulsion cross-linking. The venom's encapsulation efficiency, loading capacity, and release rate were calculated at certain time points. Moreover, the nanoparticles' optimal formulation and cytotoxic effects were determined using the MTT assay.

Results: The optimized nanoparticle formulation increases cell death induction in various cancerous cell lines. Moreover, chitosan nanoparticles loaded with Echis carinatus venom had a significant rate of cytotoxicity against cancer cells.

Conclusion: It is proposed that this formulation may act as a suitable candidate for more extensive assessments of cancer treatment using nanotechnology-based drug delivery systems.

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壳聚糖/TPP 纳米粒子的制备、表征和抗癌活性评估

目的和背景：Echis carinatus 毒液是由这种蛇的特殊腺体自然产生的一种有毒物质。除具有各种毒性外，这种毒液还具有治疗作用，可用于治疗各种癌症（肝癌、乳腺癌等）：基于纳米技术的给药系统适用于保护 Echis carinatus 毒液免受破坏和不必要的吸收。目的：基于纳米技术的给药系统适用于保护箭毒毒液免受破坏和不必要的吸收，它们可以控制毒液的转移和吸收，大大减少副作用：本研究采用离子凝胶法和乳液交联法制备壳聚糖纳米颗粒。计算了毒液在特定时间点的包封效率、负载能力和释放率。此外，还利用 MTT 试验确定了纳米颗粒的最佳配方和细胞毒性效果：结果：优化后的纳米颗粒配方可增加对各种癌细胞株的细胞死亡诱导。此外，壳聚糖纳米粒子负载的 Echis carinatus 毒液对癌细胞具有显著的细胞毒性：结论：建议将这种配方作为一种合适的候选药物，利用纳米技术药物传输系统对癌症治疗进行更广泛的评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anti-cancer agents in medicinal chemistry ONCOLOGY-CHEMISTRY, MEDICINAL

CiteScore

5.10

自引率

3.60%

发文量

323

审稿时长

4-8 weeks

期刊介绍： Formerly: Current Medicinal Chemistry - Anti-Cancer Agents. Anti-Cancer Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of anti-cancer agents. Each issue contains a series of timely in-depth reviews and guest edited issues written by leaders in the field covering a range of current topics in cancer medicinal chemistry. The journal only considers high quality research papers for publication. Anti-Cancer Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cancer drug discovery.