{"title":"Making the Most of Lateral Flow Immunochromatographic Tests: An Efficient Protocol to Recover DNA.","authors":"Sara C Zapico, Gabriela Roca","doi":"10.3390/mps7010008","DOIUrl":null,"url":null,"abstract":"<p><p>Lateral flow immunochromatographic (LFI) tests are widely used in both biomedical and forensic sciences for different applications. In forensic sciences, their main use is to detect body fluids at crime scenes. However, there are situations in which the amount of potential biological evidence is so low that DNA extraction is favored with respect to the identification of body fluids. Here, an efficient and quick protocol is presented to integrate the detection of body fluids through LFI with DNA extraction from a sample swab and buffer, providing a complete characterization of the biological evidence. This protocol is a modification of a general DNA extraction silica-based kit, whose main application is for blood and tissues. Thus, it could be carried out in different settings (forensic labs, hospitals, other testing labs) without the necessity of buying a specific kit for swabs. The validation of this protocol is supported by the results presented here and previous publications from our group, obtaining DNA in good quantity and with good quality. This proves the potential application of the protocol in both forensic scenarios, to fully characterize biological evidence, and biomedical settings, to molecularly confirm the results of LFI tests.</p>","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10801598/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/mps7010008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Lateral flow immunochromatographic (LFI) tests are widely used in both biomedical and forensic sciences for different applications. In forensic sciences, their main use is to detect body fluids at crime scenes. However, there are situations in which the amount of potential biological evidence is so low that DNA extraction is favored with respect to the identification of body fluids. Here, an efficient and quick protocol is presented to integrate the detection of body fluids through LFI with DNA extraction from a sample swab and buffer, providing a complete characterization of the biological evidence. This protocol is a modification of a general DNA extraction silica-based kit, whose main application is for blood and tissues. Thus, it could be carried out in different settings (forensic labs, hospitals, other testing labs) without the necessity of buying a specific kit for swabs. The validation of this protocol is supported by the results presented here and previous publications from our group, obtaining DNA in good quantity and with good quality. This proves the potential application of the protocol in both forensic scenarios, to fully characterize biological evidence, and biomedical settings, to molecularly confirm the results of LFI tests.
侧流免疫层析(LFI)检测被广泛应用于生物医学和法医学的不同领域。在法医学中,其主要用途是检测犯罪现场的体液。然而,在某些情况下,潜在的生物证据数量很少,因此 DNA 提取在体液鉴定中更受青睐。本文介绍了一种高效、快速的方案,可将通过 LFI 检测体液与从样本拭子和缓冲液中提取 DNA 结合起来,从而提供生物证据的完整特征。该方案是对一般 DNA 提取硅基试剂盒的改进,该试剂盒主要用于血液和组织。因此,它可以在不同的环境(法医实验室、医院、其他检测实验室)中使用,而无需购买专门用于拭子的试剂盒。本报告和我们小组以前发表的文章都证明了这一方法的有效性,我们获得了数量多、质量好的 DNA。这证明了该方案在法医领域和生物医学领域的潜在应用,前者可用于全面鉴定生物证据,后者可用于分子确认 LFI 检测结果。