Research on the Artemia nauplii microorganism compositions based on metagenomics next-generation sequencing.

IF 1.5 4区 医学 Q4 MICROBIOLOGY New Microbiologica Pub Date : 2024-01-01
Yang Xu, Dayan Hu, Haihua Cheng, Fei Peng, Qiang Gao
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Abstract

Vibrio species represent the predominant and significant pathogen in global marine fish and shellfish aquaculture. Vibrio species are ubiquitously presented in Artemia cyst hatcheries, and their notable colonization in live prey, particularly Artemia nauplii, leads to the transmission of these pathogens into the digestive system of larval organisms, causing serious problems in Vibriosis in marine aquaculture. To eliminate the Vibriospecies in Artemia nauplii, trichloroisocyanuric acid (TCCA) was used for sterilization of the nauplii. In this study, 3 different concentrations, including 0.5 ppm (FA group), 1.0 ppm (FB group) and 1.5 ppm (FC group) of TCCA were used to treat nauplii for 25 min, and then genomic DNA of the different groups were extracted followed by metagenomic next-generation sequencing (NGS). Bioinformatics analysis was applied and the results indicated that Proteobacter constituted the predominant component within each group at the phylum level, albeit accounting for only 58.68% in the FB group, which was significantly lower than in other groups (>86%). The relative abundance of Vibrio species at genus level showed that when compared with the control group, the FB group (15.8%) was reduced by 25.5%. Beta diversity showed differences between the FB group and the other groups, suggesting that treatment with 1.0ppm TCCA for 25 min would obviously reduce the Vibrio in Artemia nauplii. In conclusion, the Vibrio species were significantly reduced after treatment with TCCA, indicating that TCCA might be an alternative to antibiotics used for live food sterilization in marine aquaculture.

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基于元基因组学新一代测序技术的蒿鱼稚鱼微生物组成研究。
弧菌是全球海洋鱼类和贝类水产养殖中最主要和最重要的病原体。弧菌普遍存在于Artemia囊胚孵化场,其在活体猎物(尤其是Artemia稚鱼)中的显著定植会导致这些病原体传播到幼鱼的消化系统中,给海水养殖带来严重的弧菌病问题。为了消除笛鲷稚鳃中的弧菌,使用了三氯异氰尿酸(TCCA)对稚鳃进行消毒。本研究使用 3 种不同浓度的 TCCA 对稚虫处理 25 分钟,包括 0.5 ppm(FA 组)、1.0 ppm(FB 组)和 1.5 ppm(FC 组),然后提取不同组的基因组 DNA,进行元基因组下一代测序(NGS)。生物信息学分析结果表明,在门级水平上,蛋白菌在各组中占主要成分,但在 FB 组中仅占 58.68%,明显低于其他组(>86%)。弧菌属物种的相对丰度显示,与对照组相比,FB 组(15.8%)减少了 25.5%。Beta 多样性显示 FB 组与其他组之间存在差异,这表明用 1.0ppm TCCA 处理 25 分钟会明显减少蒿甲虫稚鱼中的弧菌。总之,经 TCCA 处理后,弧菌种类明显减少,这表明 TCCA 可替代抗生素用于海水养殖中的活体食物杀菌。
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来源期刊
New Microbiologica
New Microbiologica 生物-微生物学
CiteScore
2.20
自引率
5.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: The publication, diffusion and furtherance of research and study on all aspects of basic and clinical Microbiology and related fields are the chief aims of the journal.
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