An in-Vitro Evaluation of the Antibacterial Activity of Experimental Chlorhexidine Gluconate Solution Using Agar Diffusion Test

Abstract

The aim of this study was to investigate the antibacterial activity of three different concentrations of a newly produced CHX powder prepared loc ally in Mosul College of Dentistry in the Department of Dental Basic Sciences( approved by Nineveh Drug Industry (NDI,licence no.2256 in26/9/2002), and to compare it with the same concentrations of commercially available CHX solution against three bacterial isolates commonly isolated from infected root canals, using the agar diffusion test. Astandard agar diffusion test was utilized to investigate the antibacterial activity of different concentrations of experimental chlorhexidine gluconate solution( N DI no.2256)and acommercially available chlorhexidine solution.The diameter of the microbial inhibition zones were measured in millimeter around each paper disk containing the experimental solutions. Data were analyzed using Duncan’s Multiple Range Test (a = 0.05). The results revealed that all tested microorganisms were affected by different concentrations of CHX powder(NDI no.2256) except for the lowest concentration ( 0.1%). Similar results were observed for CHX solution. The new prepared CHX powder(NDI n.2256)can be used aroot canal irrig ant.