Development of a method for the determination of sedatives in bovine and porcine urine and kidneys by liquid chromatography–tandem mass spectrometry

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-01-20 DOI:10.2478/jvetres-2024-0001

T. Śniegocki, Elżbieta Samorek, B. Sell, Weronika Krajewska

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Abstract

Sedatives have been used for a long time as animal tranquillisers to prevent stress and weight loss during their transportation. The proper determination of these substances in food of animal origin is essential for consumer safety. A 1 g portion of pig or cow urine or homogenised kidney was mixed with acetonitrile, sodium chloride was added, and the solution was further mixed and then centrifuged. The supernatant was transferred to a new centrifuge tube with primary and secondary amine, octadecylsilane and ZrO2, and mixed rapidly. The filtered solution was evaporated under a nitrogen stream. The residue was dissolved in 200 μL of acetonitrile, centrifuged with filters and then transferred to vials. Samples were analysed by high-performance liquid chromatography–tandem mass spectrometry. The decision limit for confirmation was calculated at 2.5 μg kg−1 for all sedatives with relative standard deviation repeatability and reproducibility below 20%. The validation results showed that this method meets the pertinent EU criteria for such methods and is suitable for sedative analysis in urine and kidney matrices.

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利用液相色谱-串联质谱法开发牛和猪尿液及肾脏中镇静剂的测定方法

长期以来，镇静剂一直被用作动物的镇定剂，以防止动物在运输过程中产生应激反应和体重减轻。正确测定动物源性食品中的这类物质对消费者安全至关重要。将 1 克猪尿、牛尿或均质肾脏与乙腈混合，加入氯化钠，进一步混合后离心。将上清液转移到装有伯胺和仲胺、十八烷基硅烷和 ZrO2 的新离心管中，并迅速混合。过滤后的溶液在氮气流下蒸发。残留物溶解在 200 μL 乙腈中，用过滤器离心，然后转移到小瓶中。样品采用高效液相色谱-串联质谱法进行分析。所有镇静剂的确认判定限均为 2.5 μg kg-1，相对标准偏差重复性和再现性均低于 20%。验证结果表明，该方法符合欧盟对此类方法的相关标准，适用于尿液和肾脏基质中镇静剂的分析。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.