GC-MS characterization of Polygonatum geminiflorum depicted by antibacterial efficacy of the biosynthesized silver nanoparticles using its leaf extract

Abstract

The biological synthesis of nanomaterials is drawing immense interest because of their non-hazardous nature and enormous antimicrobial application. In the present study, we explored Polygonatum geminiflorum Decne for phytochemical profiling and biosynthesis of silver nanoparticles to control soft rot/blackleg and bacterial wilt pathogens of potato through in vitro experiment. Phytochemical screening indicated the presence of important secondary chemicals including tannins, glycosides, flavonoids and terpenoids, while, gas chromatography-mass spectrophotometry (GC-MS) study of leaf extract showed the presence of 30 phytochemicals, the most prominent among which included ç-Sitosterol and n-Hexadecanoic acid. The GC–MS qualitative analysis also supported the presence of bioactive compounds responsible for metal reduction processes and synthesized nanoparticles stabilization. In vitro study showed that concentration of 100µg/mL of AgNPs and AgNPs-PE efficiently control both Erwinia carotovora and Ralstonia solanacearum. The outcomes have provided an improved protocol to use prepared AgNPs against the tested pathogens without health hazards.