Total serum pentosidine quantification using liquid chromatography-tandem mass spectrometry

IF 2.1 Q3 ENDOCRINOLOGY & METABOLISM Bone Reports Pub Date : 2024-01-17 DOI:10.1016/j.bonr.2024.101737

Lindsie A. Blencowe , Andrea Božović , Evelyn Wong , Vathany Kulasingam , Angela M. Cheung

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Abstract

Pentosidine (PEN) is an Advanced Glycation End-product (AGE) that is known to accumulate in bone collagen with aging and contribute to fracture risk. The PEN content in bone is correlated with serum PEN, making it an attractive, potential osteoporosis biomarker. We sought to develop a method for quantifying PEN in stored serum. After conducting a systematic narrative review of PEN quantification methodologies, we developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantifying total serum PEN. Our method is both sensitive and precise (LOD 2 nM, LOQ 5 nM, %CV < 6.5 % and recovery 91.2–100.7 %). Our method is also equivalent or better than other methods identified in our review. Additionally, LC-MS/MS avoids the pitfalls and limitations of using fluorescence as a means of detection and could be adapted to investigate a broad range of AGE compounds.

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利用液相色谱-串联质谱法定量血清总戊苷

喷托西啶（PEN）是一种高级糖化终产物（AGE），已知会随着年龄的增长在骨胶原中积累，并导致骨折风险。骨骼中的 PEN 含量与血清中的 PEN 含量相关，因此 PEN 是一种有吸引力的潜在骨质疏松症生物标记物。我们试图开发一种方法来量化储存血清中的 PEN。在对 PEN 定量方法进行了系统的叙述性回顾后，我们开发了一种液相色谱串联质谱（LC-MS/MS）方法，用于定量血清中的总 PEN。我们的方法既灵敏又精确（LOD 2 nM，LOQ 5 nM，%CV < 6.5 %，回收率 91.2-100.7 %）。我们的方法也等同于或优于综述中确定的其他方法。此外，LC-MS/MS 避免了使用荧光作为检测手段的缺陷和局限性，可用于研究多种 AGE 化合物。

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来源期刊

Bone Reports Medicine-Orthopedics and Sports Medicine

CiteScore

4.30

自引率

4.00%

发文量

444

审稿时长

57 days

期刊介绍： Bone Reports is an interdisciplinary forum for the rapid publication of Original Research Articles and Case Reports across basic, translational and clinical aspects of bone and mineral metabolism. The journal publishes papers that are scientifically sound, with the peer review process focused principally on verifying sound methodologies, and correct data analysis and interpretation. We welcome studies either replicating or failing to replicate a previous study, and null findings. We fulfil a critical and current need to enhance research by publishing reproducibility studies and null findings.