Expression Profiles of Protease in Onychomycosis-Related Pathogenic Trichophyton rubrum and Tinea Capitis-Related Pathogenic Trichophyton violaceum.

IF 3.6 3区 生物学 Q2 MYCOLOGY Mycopathologia Pub Date : 2024-01-24 DOI:10.1007/s11046-024-00828-3
Jingjing Chen, Yangmin Gao, Shuzhen Xiong, Zimei Peng, Ping Zhan
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Abstract

The two fungal species Trichophyton rubrum and Trichophyton violaceum are common pathogens on human, infecting keratinized tissue of the outer body parts. Both species are belonging to the "Trichophyton rubrum complex" and share very high similarity in the genome. Secreted proteinases, key factors for keratin degradation, are nearly identical. Contrary, the ecological niches are differing. Trichophyton rubrum preferably infects skin and nails, whereas T. violaceum preferably infects the scalp. We postulate, that differences in the protease expression contribute to differences in ecological preferences. We analyzed the expression profiles of all 22 endoprotease genes, 12 subtilisins (S8A), 5 deuterolysins (M35) and 5 fungalysins (M36), for both species. To compare the influence of the keratin source, we designed experiments with human nail keratin, sheep wool keratin and keratin free cultivation media. Samples were taken at 12 h, 24 h, 48 h and 96 h post incubation in keratin medium. The expression of the proteases is higher in wool-keratin medium compared to human nail medium, with the exception of MEP4 and SUB6. Expression in the keratin-free medium is lowest. The expression profiles of the two species are remarkable different. The expression of MEP1, MEP3, SUB5, SUB11 and SUB12 are higher in T. rubrum compared to T. violaceum. MEP2, NpIIc, NpIIe, SUB1, SUB3, SUB4, SUB7 and SUB8 are higher expressed in T. violaceum compared to T. rubrum. The differences of the protease expression in the two species may expalin the differences in the ecological niches. Further analysis are necessary to verify the hypothesis.Please check and conform the edit made in title.Here I thinke the species of strains shouldnt be capital, and the right expression should be,  "Expression Profiles of Protease in Onychomycosis-Related Pathogenic Trichophyton rubrum and Tinea Capitis-Related Pathogenic Trichophyton violaceum"Author names: Please confirm if the author names are presented accurately and in the correct se-quence (given name, middle name/initial, family name). Author 1 Given name: [Jingjing] Last name [Chen], Author 2 Given name: [Yangmin] Last name [Gao], Author 3 Given name: [Shuzhen] Last name [Xiong], Author 4 Given name: [Ping] Last name [Zhan]. Also, kindly confirm the details in the metadata are correct.YesPlease check and confirm the inserted city and country are correctly identified for affiliation 3.Please change the affiliations, Affiliation 2: ²Jiangxi Provincial Clinical Research Center for Skin Diseases, Dermatology Hospital of Jiangxi Province,The Affiliated Dermatology Hospital of Nanchang University, Nanchang, 330200, Jiangxi; Affiliation 3: 3Institute of Clinical Medicine, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College,Nanchang 330001, Jiangxi. Thanks a lot!

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与甲癣相关的致病性红色毛癣菌和与头癣相关的致病性毛癣菌中蛋白酶的表达谱。
红毛癣菌(Trichophyton rubrum)和暴力毛癣菌(Trichophyton violaceum)这两种真菌是人类常见的病原体,会感染身体外部的角质化组织。这两种真菌都属于 "红色毛癣菌复合体",其基因组具有极高的相似性。作为角蛋白降解关键因素的分泌蛋白酶也几乎相同。相反,它们的生态位却不同。红色毛癣菌喜欢感染皮肤和指甲,而暴力毛癣菌则喜欢感染头皮。我们推测,蛋白酶表达的差异导致了生态偏好的不同。我们分析了这两个物种的全部 22 个内切蛋白酶基因的表达谱,包括 12 个枯草蛋白酶(S8A)、5 个脱氧溶酶(M35)和 5 个真菌溶酶(M36)。为了比较角蛋白来源的影响,我们设计了使用人类指甲角蛋白、羊毛角蛋白和无角蛋白培养基的实验。在角蛋白培养基中培养 12 小时、24 小时、48 小时和 96 小时后取样。与人指甲培养基相比,羊毛角蛋白培养基中蛋白酶的表达量更高,但 MEP4 和 SUB6 除外。无角蛋白培养基中的表达量最低。两个物种的表达谱差异显著。与 T. violaceum 相比,MEP1、MEP3、SUB5、SUB11 和 SUB12 在 T. rubrum 中的表达量更高。与 T. rubrum 相比,MEP2、NpIIc、NpIIe、SUB1、SUB3、SUB4、SUB7 和 SUB8 在 T. violaceum 中的表达量更高。两个物种蛋白酶表达的差异可能说明了生态位的不同。请检查并修改标题中的编辑内容,我认为菌株的种类不应大写,正确的表述应该是:"Expression Profiles of Protease in Onychomycosis-Related Pathogenic Trichophyton rubrum and Tinea Capitis-Related Pathogenic Trichophyton violaceum":请确认作者姓名是否准确,顺序是否正确(名、中名/姓、姓)。作者 1 名:[晶晶] 姓:[陈],作者 2 名:[杨敏] 姓:[高],作者 3 名:[淑珍] 姓:[熊],作者 4 名:[平] 姓:[詹]。此外,请确认元数据中的详细信息正确无误。是请检查并确认所插入的城市和国家是否正确标识为隶属关系 3。请更改所属单位,所属单位 2:²江西省皮肤病临床研究中心,江西省皮肤病医院,南昌大学附属皮肤病医院,江西南昌,330200;所属单位 3:3江西省人民医院临床医学研究所,南昌医学院第一附属医院,江西南昌,330001。非常感谢
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来源期刊
Mycopathologia
Mycopathologia 生物-真菌学
CiteScore
6.80
自引率
3.60%
发文量
76
审稿时长
3 months
期刊介绍: Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.
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