THAP9-AS1 promotes nasopharyngeal carcinoma progression through targeted regulation of the miR-185-5p/SOX13 axis.

IF 2.2 4区医学 Q3 PHYSIOLOGY Physiology international Pub Date : 2024-01-25 Print Date: 2024-03-21 DOI:10.1556/2060.2023.00232

Jinhua Mo, Zengyi Gong, Hong Liu, Lian Zhou, Yanguang Zhao

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Abstract

Background: It has been reported that long non-coding RNA THAP9-AS1 exerts carcinogenic role by mediating miRNAs and target genes in various human cancers. However, whether THAP9-AS1 influences the progression of nasopharyngeal carcinoma (NPC) remains unknown.

Methods: The transcriptional levels of THAP9-AS1 and miR-185-5p were estimated via quantitative real time polymerase chain reaction (qRT-PCR) assay. The protein level of SOX13 was detected with western blotting assay. Additionally, methyl thiazolyl tetrazolium (MTT) assay as well as colony formation assay were utilized to measure cell growth. The apoptotic cells were observed by employing Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) staining analysis, and transwell assay was introduced to test cell migration in addition to invasion. Moreover, the relationship between miR-185-5p and THAP9-AS1 or SOX13 was estimated through dual-luciferase reporter gene assay.

Results: THAP9-AS1 was overexpressed in head and neck squamous cell carcinoma (HNSCC) tissues and NPC cells. Besides, silencing of THAP9-AS1 depressed the life processes of NPC cells including cell growth, migration as well as invasion but facilitated cell apoptosis. Further investigation proved that miR-185-5p was the direct target of THAP9-AS1. Besides, the knockdown of THAP9-AS1 notably reduced the transcriptional level of miR-185-5p. Furthermore, THAP9-AS1 served as a sponge of miR-185-5p to modulate the expression of SOX13, which regulated the development of NPC cells.

Conclusion: This work verified that THAP9-AS1 promoted NPC cell progression at least partly by mediating the miR-185-5p/SOX13 axis.

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THAP9-AS1 通过靶向调控 miR-185-5p/SOX13 轴促进鼻咽癌的进展。

背景：有报道称，长非编码RNA THAP9-AS1在多种人类癌症中通过介导miRNA和靶基因发挥致癌作用。然而，THAP9-AS1 是否会影响鼻咽癌（NPC）的进展仍是未知数：方法：通过定量实时聚合酶链反应（qRT-PCR）测定 THAP9-AS1 和 miR-185-5p 的转录水平。SOX13的蛋白水平通过Western印迹法检测。此外，还利用甲基噻唑基四氮唑（MTT）检测法和菌落形成检测法测量细胞生长情况。采用末端脱氧核苷酸转移酶介导的尼克末端标记（TUNEL）染色分析法观察细胞凋亡情况，并采用透孔试验检测细胞迁移和侵袭情况。此外，还通过双荧光素酶报告基因检测法评估了 miR-185-5p 与 THAP9-AS1 或 SOX13 的关系：结果：THAP9-AS1在头颈部鳞状细胞癌（HNSCC）组织和鼻咽癌细胞中过表达。此外，沉默 THAP9-AS1 会抑制鼻咽癌细胞的生命过程，包括细胞生长、迁移和侵袭，但会促进细胞凋亡。进一步研究证明，miR-185-5p 是 THAP9-AS1 的直接靶标。此外，敲除 THAP9-AS1 会显著降低 miR-185-5p 的转录水平。此外，THAP9-AS1作为miR-185-5p的海绵，可调节SOX13的表达，从而调控鼻咽癌细胞的发育：这项研究证实，THAP9-AS1至少在一定程度上通过介导miR-185-5p/SOX13轴促进了鼻咽癌细胞的发展。

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来源期刊

Physiology international Medicine-Physiology (medical)

CiteScore

3.40

自引率

0.00%

发文量

期刊介绍： The journal provides a forum for important new research papers written by eminent scientists on experimental medical sciences. Papers reporting on both original work and review articles in the fields of basic and clinical physiology, pathophysiology (from the subcellular organization level up to the oranizmic one), as well as related disciplines, including history of physiological sciences, are accepted.