{"title":"Influence of pre-B cell receptor deficiency on the immunoglobulin repertoires in peripheral blood B cells before and after immunization","authors":"Takeyuki Shimizu , Lin Sun , Kazuo Ohnishi","doi":"10.1016/j.molimm.2024.01.001","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span>During B cell development, pre-B cell receptor (pre-BCR), comprising the </span>immunoglobulin heavy chain (HC) and </span>surrogate light chain<span> (SLC), plays a crucial role. The expression of pre-BCR serves as a certification of HC quality, confirming its ability to associate with the SLC and light chain (LC). In mice lacking SLC, the absence of this quality control mechanism leads to a distorted repertoire of HCs in the spleen and bone marrow. In this study, we conducted a comparative analysis of the immunoglobulin gene repertoire in peripheral </span></span>blood cells<span><span> of both wild-type mice and pre-BCR–deficient mice. Our findings reveal differences not only in the μ HC repertoire but also in the α HC and κ LC repertoires of the pre-BCR–deficient mice. These results suggest that the pre-BCR–mediated quality check of HC influences the selection of class-switched HC and LC repertoires. To further explore the impact of pre-BCR deficiency, we immunized these mice with thymus-dependent antigens and compared the antigen-responding repertoires. Our observations indicate that the </span>affinity maturation pathways remain consistent between wild-type mice and pre-BCR–deficient mice, albeit with variations in the degree of maturation.</span></p></div>","PeriodicalId":18938,"journal":{"name":"Molecular immunology","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular immunology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0161589024000038","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
During B cell development, pre-B cell receptor (pre-BCR), comprising the immunoglobulin heavy chain (HC) and surrogate light chain (SLC), plays a crucial role. The expression of pre-BCR serves as a certification of HC quality, confirming its ability to associate with the SLC and light chain (LC). In mice lacking SLC, the absence of this quality control mechanism leads to a distorted repertoire of HCs in the spleen and bone marrow. In this study, we conducted a comparative analysis of the immunoglobulin gene repertoire in peripheral blood cells of both wild-type mice and pre-BCR–deficient mice. Our findings reveal differences not only in the μ HC repertoire but also in the α HC and κ LC repertoires of the pre-BCR–deficient mice. These results suggest that the pre-BCR–mediated quality check of HC influences the selection of class-switched HC and LC repertoires. To further explore the impact of pre-BCR deficiency, we immunized these mice with thymus-dependent antigens and compared the antigen-responding repertoires. Our observations indicate that the affinity maturation pathways remain consistent between wild-type mice and pre-BCR–deficient mice, albeit with variations in the degree of maturation.
在 B 细胞发育过程中,由免疫球蛋白重链(HC)和替代轻链(SLC)组成的前 B 细胞受体(pre-BCR)起着至关重要的作用。前 BCR 的表达可作为 HC 质量的证明,确认其与 SLC 和轻链(LC)结合的能力。在缺乏 SLC 的小鼠中,这种质量控制机制的缺失会导致脾脏和骨髓中的 HC 出现扭曲。在这项研究中,我们对野生型小鼠和前 BCR 缺陷小鼠外周血细胞中的免疫球蛋白基因库进行了比较分析。我们的研究结果表明,前 BCR 基因缺陷小鼠不仅在 μ HC 基因库中存在差异,而且在 α HC 和 κ LC 基因库中也存在差异。这些结果表明,前 BCR 介导的 HC 质量检查影响了类开关 HC 和 LC 重奏的选择。为了进一步探讨前BCR缺陷的影响,我们用胸腺依赖性抗原对这些小鼠进行了免疫,并比较了抗原反应序列。我们的观察结果表明,野生型小鼠和前BCR缺陷小鼠的亲和力成熟途径保持一致,只是成熟程度有所不同。
期刊介绍:
Molecular Immunology publishes original articles, reviews and commentaries on all areas of immunology, with a particular focus on description of cellular, biochemical or genetic mechanisms underlying immunological phenomena. Studies on all model organisms, from invertebrates to humans, are suitable. Examples include, but are not restricted to:
Infection, autoimmunity, transplantation, immunodeficiencies, inflammation and tumor immunology
Mechanisms of induction, regulation and termination of innate and adaptive immunity
Intercellular communication, cooperation and regulation
Intracellular mechanisms of immunity (endocytosis, protein trafficking, pathogen recognition, antigen presentation, etc)
Mechanisms of action of the cells and molecules of the immune system
Structural analysis
Development of the immune system
Comparative immunology and evolution of the immune system
"Omics" studies and bioinformatics
Vaccines, biotechnology and therapeutic manipulation of the immune system (therapeutic antibodies, cytokines, cellular therapies, etc)
Technical developments.