{"title":"Dynamic changes of lipids in Eucommia ulmoides rubber particles and the relationship between the biosynthesis of SQDG and E. ulmoides rubber","authors":"Chuhan Zhang, Ting Yuan, Lijun Qin","doi":"10.1007/s42464-023-00232-4","DOIUrl":null,"url":null,"abstract":"<div><p><i>Eucommia ulmoides</i> rubber particles (EuRPs) are organelles that synthesise and store <i>E. ulmoides</i> rubber (EuR). In this study, the lipids dynamic change of EuRPs from leaf tissue at different growth period were investigated. The results showed that there were 1149 lipid species belonging to 39 lipid classes that were identified; TGs, DGs and Cers were the most abundant among all lipids of EuRPs. Lipid expression analysis demonstrated that the expression intensity of TGs was almost 1 × 10<sup>4</sup>-fold and 10-fold higher than those of MGs and DGs, respectively. Among sphingolipids, the expression intensity of SoP, CerG2, GM3, SM and GerG3 increased throughout the duration of investigation (August–November 2021). Further analysis found that the Cers species accounted for 9.40% of total lipid species and the number of Cers species in EuRPs isolated from leaves in August was the most, with gradual decline in the following 3 months. Meanwhile, hierarchical clustering indicated that there were seven types of highly abundant lipid species in leaf EuRPs from September to November, i.e<i>.</i> SQDG (18:3/18:3), SQDG (34:3), SQDG (17:5/23:2), DGDG (38:12), Co (Q9), TG (16:0/16:0/18:3) and TG (39:1). Compared with leaf EuRPs in August, there were 79, 101 and 107 lipid species which showed significant differential expression in September, October and November. Based on the high expression intensity of SQDG in EuRPs, the correlation between the genes mediated in EuR or SQDG biosynthesis was further analysed, whereby the results indicated co-expression of some genes, which implies the synergistic regulatory role in SQDG EuR biosynthesis.</p></div>","PeriodicalId":662,"journal":{"name":"Journal of Rubber Research","volume":"27 1","pages":"33 - 46"},"PeriodicalIF":1.2000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Rubber Research","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s42464-023-00232-4","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Eucommia ulmoides rubber particles (EuRPs) are organelles that synthesise and store E. ulmoides rubber (EuR). In this study, the lipids dynamic change of EuRPs from leaf tissue at different growth period were investigated. The results showed that there were 1149 lipid species belonging to 39 lipid classes that were identified; TGs, DGs and Cers were the most abundant among all lipids of EuRPs. Lipid expression analysis demonstrated that the expression intensity of TGs was almost 1 × 104-fold and 10-fold higher than those of MGs and DGs, respectively. Among sphingolipids, the expression intensity of SoP, CerG2, GM3, SM and GerG3 increased throughout the duration of investigation (August–November 2021). Further analysis found that the Cers species accounted for 9.40% of total lipid species and the number of Cers species in EuRPs isolated from leaves in August was the most, with gradual decline in the following 3 months. Meanwhile, hierarchical clustering indicated that there were seven types of highly abundant lipid species in leaf EuRPs from September to November, i.e. SQDG (18:3/18:3), SQDG (34:3), SQDG (17:5/23:2), DGDG (38:12), Co (Q9), TG (16:0/16:0/18:3) and TG (39:1). Compared with leaf EuRPs in August, there were 79, 101 and 107 lipid species which showed significant differential expression in September, October and November. Based on the high expression intensity of SQDG in EuRPs, the correlation between the genes mediated in EuR or SQDG biosynthesis was further analysed, whereby the results indicated co-expression of some genes, which implies the synergistic regulatory role in SQDG EuR biosynthesis.
期刊介绍：
The Journal of Rubber Research is devoted to both natural and synthetic rubbers, as well as to related disciplines. The scope of the journal encompasses all aspects of rubber from the core disciplines of biology, physics and chemistry, as well as economics. As a specialised field, rubber science includes within its niche a vast potential of innovative and value-added research areas yet to be explored. This peer reviewed publication focuses on the results of active experimental research and authoritative reviews on all aspects of rubber science.
The Journal of Rubber Research welcomes research on:
the upstream, including crop management, crop improvement and protection, and biotechnology;
the midstream, including processing and effluent management;
the downstream, including rubber engineering and product design, advanced rubber technology, latex science and technology, and chemistry and materials exploratory;
economics, including the economics of rubber production, consumption, and market analysis.
The Journal of Rubber Research serves to build a collective knowledge base while communicating information and validating the quality of research within the discipline, and bringing together work from experts in rubber science and related disciplines.
Scientists in both academia and industry involved in researching and working with all aspects of rubber will find this journal to be both source of information and a gateway for their own publications.
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