Investigating the Characteristics of Nanoliposomes Carrying Bioactive Peptides Obtained from Shrimp Waste

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-01-24 DOI:10.1007/s10989-024-10587-w

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Abstract

The aim of the paper was to develop chitosan-coated nanoliposomes for carrying bioactive peptides derived from hydrolyzed shrimp waste (SW) with chitosan coating (concentrations of 0, 0.5 and 1% W/ V). The study involved producing SW hydrolysates using protease enzymes from Bacillus salsus bacteria, followed by investigating the physicochemical, antioxidant, and antimicrobial properties of the peptide-loaded nanoliposomes. The dispersion index ranged from 0.17 to 0.65, and the particle size varied from 500 to 685 nm. Increasing the chitosan concentration to 1% significantly increased the particle size (P < 0.05). The Zeta potential of the nanoliposomes became positive as the chitosan concentration increased, starting from − 47.73 mV and reaching + 36.40 mV. The highest encapsulation efficiency (84.67%) was observed in nanoliposomes with 1% chitosan, while uncoated liposomes had the lowest encapsulation efficiency (44%). Scanning electron microscopy (SEM) revealed that the nanoparticles exhibited homogeneous, spherical, and cluster-shaped structures with smooth surfaces. Chitosan coating enhanced the stability of the peptides in simulated stomach and intestinal environments, with higher stability observed at higher chitosan concentrations (P < 0.05). Chitosan-coated nanoliposomes exhibited higher antioxidant and antimicrobial activities compared to uncoated nanoliposomes. Increasing the chitosan concentration resulted in greater inhibition of free radicals (DPPH and ABTS free radical activities increased significantly in nanoliposomes with 1% chitosan compared to uncoated samples: 32.56–70.28% and 41.58–80.28% respectively) and enhanced antimicrobial properties (P < 0.05). In conclusion, coating hydrolyzed SW with nanoliposomes and chitosan improved the structural, physicochemical, antioxidant, and antimicrobial properties of the peptides, making them suitable for direct application in food products requiring antioxidant and antimicrobial effects.

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研究从虾废料中提取的携带生物活性肽的纳米脂质体的特性

摘要本文旨在开发壳聚糖涂层纳米脂质体，用于携带从水解虾废料（SW）中提取的生物活性肽。该研究利用沙氏芽孢杆菌的蛋白酶生产虾废料水解物，然后研究了载肽纳米脂质体的理化、抗氧化和抗菌特性。分散指数在 0.17 至 0.65 之间，粒径在 500 至 685 纳米之间。将壳聚糖浓度提高到 1%，粒径会明显增大（P < 0.05）。随着壳聚糖浓度的增加，纳米脂质体的 Zeta 电位从 - 47.73 mV 变为 + 36.40 mV。含有 1%壳聚糖的纳米脂质体的包封效率最高（84.67%），而未包封脂质体的包封效率最低（44%）。扫描电子显微镜（SEM）显示，纳米颗粒呈现出均匀、球形和簇状结构，表面光滑。壳聚糖涂层增强了多肽在模拟胃肠环境中的稳定性，壳聚糖浓度越高，稳定性越高（P < 0.05）。与未包被的纳米脂质体相比，包被壳聚糖的纳米脂质体具有更高的抗氧化和抗菌活性。增加壳聚糖的浓度可提高对自由基的抑制作用（与未包被样品相比，包被 1%壳聚糖的纳米脂质体的 DPPH 和 ABTS 自由基活性显著增加：分别为 32.56-70.28% 和 41.58-80.28%），并增强了抗菌性能（P < 0.05）。总之，用纳米脂质体和壳聚糖包覆水解SW可改善肽的结构、理化、抗氧化和抗菌特性，使其适合直接应用于需要抗氧化和抗菌效果的食品中。

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