A Potentiometric Dual-Channel Microsensor Reveals that Fluctuation of H2S is Less pH-Dependent During Spreading Depolarization in the Rat Brain

IF 16.1 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Angewandte Chemie International Edition Pub Date : 2024-01-25 DOI:10.1002/anie.202318973

Rantong Liu, Shuai Zhang, Hui Zeng, Nan Gao, Yongyue Yin, Prof. Meining Zhang, Prof. Lanqun Mao

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Abstract

Spreading depolarization (SD) is one of the most common neuropathologic phenomena in the nervous system, relating to numerous diseases. However, real-time monitoring the rapid chemical changes during SD to probe the molecular mechanism remains a great challenge. We develop a potentiometric dual-channel microsensor for simultaneous monitoring of H₂S and pH featuring excellent selectivity and spatiotemporal resolution. Using this microsensor we first observe real time changes of H₂S and pH in the rat brain induced by SD. This changes of H₂S are completely suppressed when the rat pre-treats with aminooxyacetic acid (AOAA), a blocker to inhibit the H₂S-producing enzyme, indicating H₂S fluctuation might be related to enzyme-dependent pathway during SD and less pH-dependent. This study provides a new perspective for studying the function of H₂S and the molecular basis of SD-associated diseases.

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电位计双通道微传感器揭示了在大鼠大脑的扩展性去极化过程中，H2S 的波动对 pH 值的依赖性较小。

扩散性去极化（SD）是神经系统中最常见的神经病理现象之一，与多种疾病相关。然而，实时监测 SD 期间的快速化学变化以探究其分子机制仍然是一个巨大的挑战。我们开发了一种同时监测 H2S 和 pH 值的电位双通道微传感器，它具有出色的选择性和时空分辨率。利用这种微型传感器，我们首次观测到了 SD 诱导的大鼠大脑中 H2S 和 pH 的实时变化。当大鼠使用氨基氧乙酸（AOAA）（一种抑制 H2S 生成酶的阻断剂）进行预处理时，H2S 的变化被完全抑制。这项研究为研究H2S的功能和SD相关疾病的分子基础提供了一个新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Angewandte Chemie International Edition 化学-化学综合

CiteScore

26.60

自引率

6.60%

发文量

3549

审稿时长

1.5 months

期刊介绍： Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.