PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkolderia cenocepacia

IF 6.1 1区 医学 Q1 PHARMACOLOGY & PHARMACY Journal of Pharmaceutical Analysis Pub Date : 2024-01-26 DOI:10.1016/j.jpha.2024.01.007
Tinatini Tchatchiashvilli, Helena Duering, Lisa Mueller-Boetticher, Christian Grune, Dagmar Fischer, Mathias W. Pletz, Oliwia Makarewicz
{"title":"PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkolderia cenocepacia","authors":"Tinatini Tchatchiashvilli, Helena Duering, Lisa Mueller-Boetticher, Christian Grune, Dagmar Fischer, Mathias W. Pletz, Oliwia Makarewicz","doi":"10.1016/j.jpha.2024.01.007","DOIUrl":null,"url":null,"abstract":"<p>In our prior research, polymer nanoparticles containing tobramycin displayed robust antibacterial efficacy against biofilm-embedded <em>Pseudomonas aeruginosa</em> and <em>Burkholderia cenocepacia</em> cells, critical pathogens in cystic fibrosis. In the current study, we investigated the deposition of a nanoparticulate carrier composed of poly(D,L-lactic-<em>co</em>-glycolic acid) (PLGA) and poly(ethylene glycol)-<em>block</em>-PLGA (PEG-PLGA) that was either covalently bonded with cyanine-5-amine or noncovalently bound with freely embedded cationic rhodamine B, which served as a drug surrogate. After exposing these nanoparticles to bacteria, we performed cell fractionation and fluorescence analysis, which highlighted the accumulation of cyanine-5-amine in the outer membranes and the accumulation of rhodamine B in the cytoplasm of cells. The results indicated that these organic nanoparticles are effective vehicles for targeted antibiotic delivery in bacterial cells, explaining the observed increase in the efficacy of encapsulated tobramycin against biofilms. This work emphasizes the potential of PEG-PLGA-based formulations for advanced drug delivery strategies.</p>","PeriodicalId":16737,"journal":{"name":"Journal of Pharmaceutical Analysis","volume":"3 1","pages":""},"PeriodicalIF":6.1000,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmaceutical Analysis","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jpha.2024.01.007","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0

Abstract

In our prior research, polymer nanoparticles containing tobramycin displayed robust antibacterial efficacy against biofilm-embedded Pseudomonas aeruginosa and Burkholderia cenocepacia cells, critical pathogens in cystic fibrosis. In the current study, we investigated the deposition of a nanoparticulate carrier composed of poly(D,L-lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol)-block-PLGA (PEG-PLGA) that was either covalently bonded with cyanine-5-amine or noncovalently bound with freely embedded cationic rhodamine B, which served as a drug surrogate. After exposing these nanoparticles to bacteria, we performed cell fractionation and fluorescence analysis, which highlighted the accumulation of cyanine-5-amine in the outer membranes and the accumulation of rhodamine B in the cytoplasm of cells. The results indicated that these organic nanoparticles are effective vehicles for targeted antibiotic delivery in bacterial cells, explaining the observed increase in the efficacy of encapsulated tobramycin against biofilms. This work emphasizes the potential of PEG-PLGA-based formulations for advanced drug delivery strategies.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
PEG-PLGA 纳米颗粒沉积在铜绿假单胞菌和芽孢杆菌中
在我们之前的研究中,含有妥布霉素的聚合物纳米颗粒对生物膜包埋的铜绿假单胞菌和伯克霍尔德氏球菌细胞(囊性纤维化的重要病原体)显示出强大的抗菌功效。在当前的研究中,我们研究了一种由聚(D,L-乳酸-共聚乙醇酸)(PLGA)和聚(乙二醇)-嵌段-PLGA(PEG-PLGA)组成的纳米颗粒载体的沉积,该载体与作为药物替代物的氰基-5-胺共价结合或与自由嵌入的阳离子罗丹明 B 非共价结合。将这些纳米粒子暴露于细菌后,我们进行了细胞分馏和荧光分析,结果表明氰基-5-胺在细胞外膜聚集,而罗丹明 B 则在细胞质中聚集。结果表明,这些有机纳米粒子是在细菌细胞中靶向递送抗生素的有效载体,这也是所观察到的封装妥布霉素对生物膜疗效提高的原因。这项研究强调了基于 PEG-PLGA 的制剂在先进给药策略方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Pharmaceutical Analysis
Journal of Pharmaceutical Analysis Chemistry-Electrochemistry
CiteScore
16.20
自引率
2.30%
发文量
674
审稿时长
22 weeks
期刊介绍: The Journal of Pharmaceutical Analysis (JPA), established in 2011, serves as the official publication of Xi'an Jiaotong University. JPA is a monthly, peer-reviewed, open-access journal dedicated to disseminating noteworthy original research articles, review papers, short communications, news, research highlights, and editorials in the realm of Pharmacy Analysis. Encompassing a wide spectrum of topics, including Pharmaceutical Analysis, Analytical Techniques and Methods, Pharmacology, Metabolism, Drug Delivery, Cellular Imaging & Analysis, Natural Products, and Biosensing, JPA provides a comprehensive platform for scholarly discourse and innovation in the field.
期刊最新文献
Hepatic protein phosphatase 1 regulatory subunit 3G alleviates obesity and liver steatosis by regulating the gut microbiota and bile acid metabolism Retraction notice to “A DNA-based nanocarrier for efficient cancer therapy” [J. Pharm. Anal. 11 (2021) 330–339] Mapping conformational changes on bispecific antigen-binding biotherapeutic by covalent labeling and mass spectrometry Medcheck: a novel software for automatic de-formulation of traditional Chinese medicine (TCM) prescriptions by liquid chromatography-mass spectrometry 17β-Estradiol, through activating the G protein-coupled estrogen receptor, exacerbates the complication of benign prostate hyperplasia in type 2 diabetes mellitus patients by inducing prostate proliferation
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1