Inactivation of SARS-CoV-2 in serum using physical methods

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES Japanese journal of infectious diseases Pub Date : 2024-01-31 DOI:10.7883/yoken.jjid.2023.349
Toshihiko Harada, Hirofumi Fujimoto, Shuetsu Fukushi, Koji Ishii, Ken-Ichi Hanaki
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Abstract

Since 2019, many studies on COVID-19, which has caused extensive damage as a pandemic, have been ongoing worldwide. These include serological and biochemical studies using sera from patients and animal models. Testing with these sera must be performed after the inactivation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Heat treatment, UV irradiation, and/or gamma-ray irradiation are used to inactivate viruses in serum. Determining the inactivation conditions that ensure the inactivation of viruses and minimize the effect on test results after inactivation is important to ensure worker safety and accuracy of test results. In this study, serum samples containing SARS-CoV-2 were subjected to heat, UV irradiation, and gamma irradiation to determine their inactivation conditions. The viral titers were below the detection limit after heating at 56°C for 1 h or 60°C for 15 min, UV-B irradiation with a transilluminator for 30 min, or gamma ray irradiation with 60Co at 10 kGy. These results provide useful information for safe serological and biochemical experiments.

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用物理方法使血清中的 SARS-CoV-2 失活
COVID-19 作为一种大流行病造成了广泛的破坏,自 2019 年以来,世界各地一直在对 COVID-19 进行多项研究。这些研究包括使用患者和动物模型血清进行的血清学和生化研究。对这些血清的检测必须在严重急性呼吸系统综合症冠状病毒 2(SARS-CoV-2)灭活后进行。热处理、紫外线照射和/或伽马射线照射可用于灭活血清中的病毒。确定灭活条件以确保灭活病毒并将灭活后对检测结果的影响降至最低,对于确保工人的安全和检测结果的准确性非常重要。本研究对含有 SARS-CoV-2 的血清样本进行了加热、紫外线照射和伽马射线照射,以确定其灭活条件。在 56°C 加热 1 小时或 60°C 加热 15 分钟、用透射照相机照射紫外线-B 30 分钟或用 10 kGy 的 60Co 伽马射线照射后,病毒滴度均低于检测限。这些结果为安全的血清学和生化实验提供了有用的信息。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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