Improving linolenic acid content in rapeseed oil by overexpression of CsFAD2 and CsFAD3 genes

IF 2.6 3区农林科学 Q1 AGRONOMY Molecular Breeding Pub Date : 2024-01-29 DOI:10.1007/s11032-024-01445-0

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Abstract

With the increasing public attention to the health benefit of polyunsaturated fatty acids (PUFAs) and demand for linolenic acid (C18:3), it is of great significance to increase the C18:3 content in our meal. As an oil crop with high content of C18:3, Camelina sativa has three homologous copies of FAD2 and three homologous copies FAD3. In this study, we seed-specifically overexpressed two Camelina sativa fatty acid desaturase genes, CsFAD2 and CsFAD3, in rapeseed cultivar Zhongshuang 9. The results show that C18:3 content in CsFAD2 and CsFAD3 overexpressed seeds is increased from 8.62% in wild-type (WT) to 10.62–12.95% and 14.54–26.16%, respectively. We crossed CsFAD2 and CsFAD3 overexpression lines, and stable homozygous digenic crossed lines were obtained. The C18:3 content was increased from 8.62% in WT to 28.46–53.57% in crossed overexpression lines. In addition, we found that the overexpression of CsFAD2 and CsFAD3 had no effect on rapeseed growth, development, and other agronomic traits. In conclusion, we successfully generated rapeseed germplasms with high C18:3 content by simultaneously overexpressing CsFAD2 and CsFAD3, which provides a feasible way for breeding high C18:3 rapeseed cultivars.

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通过过表达 CsFAD2 和 CsFAD3 基因提高菜籽油中亚麻酸的含量

摘要随着公众对多不饱和脂肪酸（PUFA）健康益处的日益关注以及对亚麻酸（C18:3）需求的增加，提高膳食中的 C18:3 含量意义重大。作为一种 C18:3 含量较高的油料作物，荠菜有三个 FAD2 同源拷贝和三个 FAD3 同源拷贝。本研究在油菜品种中双 9 号中特异性过表达了两个荠菜脂肪酸去饱和酶基因 CsFAD2 和 CsFAD3。结果表明，CsFAD2 和 CsFAD3 过表达种子中的 C18:3 含量分别从野生型（WT）的 8.62% 增加到 10.62-12.95% 和 14.54-26.16%。我们对 CsFAD2 和 CsFAD3 过表达株系进行杂交，获得了稳定的同源二系杂交株系。杂交过表达株的 C18:3 含量从 WT 的 8.62% 提高到 28.46-53.57%。此外，我们发现 CsFAD2 和 CsFAD3 的过表达对油菜籽的生长、发育和其他农艺性状没有影响。总之，通过同时过表达 CsFAD2 和 CsFAD3，我们成功地培育出了高 C18:3 含量的油菜种质，为培育高 C18:3 油菜品种提供了一条可行的途径。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.