Investigation of the presence of equine herpesvirus-1 (EHV-1) in tissue samples of aborted foals by histopathological, immunohistochemical, and Real-Time PCR techniques

Pub Date : 2024-01-18 DOI:10.12681/jhvms.34093

Sezgin Deniz, Ahmet Gülçubuk, K. Sönmez, E. Altan, Işıl Aytemiz Danyer, Aydın Gürel, Hüseyin Yılmaz

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Abstract

Equine herpesvirus-1 (EHV-1) is a cause of pneumonia, encephalitis, and abortion in horses. Abortions occur alive or dead in the last trimester of pregnancy. The aim of this study is to investigate the incidence of EHV-1 infections in the tissue samples of aborted stillborn and live-born foals and the foals which died soon after birth and compare and correlate the conventional histopathologic diagnosis with IHC and Real-Time PCR. For this purpose, tissue samples (lung, liver, spleen, kidney, placenta, and brain) of 38 foals from Istanbul, Kocaeli, Bursa, and Eskişehir provinces of Turkey between August 2009 and March 2011 were examined Real-Time PCR revealed 52.6 % positivity (20/38), whereas 39.4 % (15/38) positivity was achieved with IHC, which indicated a correlation between these two diagnostic methods. In histopathological evaluation, the inclusion body was found in at least one of the tissue sections belonging to the lung, spleen, and mostly liver of 20 foals (52.6%). Tissue samples of the foals found to be positive by IHC and PCR revealed histopathologic findings compatible with the characteristics of the disease.

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通过组织病理学、免疫组织化学和实时 PCR 技术研究流产马驹组织样本中是否存在马疱疹病毒-1 (EHV-1)

马疱疹病毒-1（EHV-1）可导致马匹肺炎、脑炎和流产。流产发生在妊娠的最后三个月，或死或活。本研究的目的是调查流产死胎、活产马驹和出生后不久死亡的马驹的组织样本中 EHV-1 感染的发生率，并将传统的组织病理学诊断与 IHC 和 Real-Time PCR 进行比较和关联。为此，研究人员对 2009 年 8 月至 2011 年 3 月期间来自土耳其伊斯坦布尔、科贾埃利、布尔萨和埃斯基谢希尔省的 38 只小马驹的组织样本（肺、肝、脾、肾、胎盘和脑）进行了检测，结果显示，Real-Time PCR 阳性率为 52.6%（20/38），而 IHC 阳性率为 39.4%（15/38），这表明这两种诊断方法之间存在相关性。在组织病理学评估中，20 头小马驹（52.6%）的肺部、脾脏和大部分肝脏组织切片中至少有一处发现了包涵体。经 IHC 和 PCR 检测呈阳性的马驹组织样本显示的组织病理学结果与该疾病的特征相符。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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