The Protective Role of Vitamin C on Heamatological (WBC and Hb) Alterations in Commom Carp During Chronic Toxicity of Lead Nitrate

Musrat Majeed, Rajendra Chauhan, Tehleel Ahmed
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Abstract

This investigation intended to assess the outcome of haematological factors (WBCs and Hb) on common carp (Cyprinus carpio) of weight around (80 – 120 g) being exposed to various doses of lead nitrate and the protective role of Vitamin C in response to lead nitrate changes were studied. Chemicals (or elements) known as heavy metals are discharged into the surrounding environment as a consequence of commercial and industrial use as well as leaks from landfills. They are water-soluble but not biodegradable, and they are frequently firmly bound to proteins and polypeptides, even if previous research has issued warnings. The aquatic ecosystem is still impacted by several activities, changing the temperature and posing health risks to fish. The aquatic ecology becomes unbalanced as a result of heavy metals. Several lead nitrate concentrations were applied to the fish, and after 24, 48, 72, and 96 hours, (percentage)% mortality was recorded. Throughout the current experimental period, seven distinct concentrations of lead nitrate was used to determine the LC50 of the substance and evaluate its effect on common carp mortality. The lead nitrate concentrations ranged between 10 and 60 mg/l, and each concentration's death rate was determined. Lead nitrate was found to have an LC100 of 60 mg/l. Therefore, it was determined that the acute 96-hour LC50 value for the common carp, the fish used in this experiment, was 35 mg/l (ppm).
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维生素 C 对硝酸铅慢性中毒期间鲤鱼血液学(白细胞和血红蛋白)变化的保护作用
这项调查旨在评估体重在 80 - 120 克左右的鲤鱼(Cyprinus carpio)暴露于不同剂量硝酸铅后的血液学因素(白细胞和血红蛋白)的结果,并研究维生素 C 对硝酸铅变化的保护作用。被称为重金属的化学品(或元素)由于商业和工业用途以及垃圾填埋场的泄漏而被排放到周围环境中。它们可溶于水,但不可生物降解,而且经常与蛋白质和多肽牢固结合,即使以前的研究已经发出过警告。水生生态系统仍然受到多种活动的影响,温度发生变化,对鱼类的健康造成危害。重金属导致水生生态失衡。对鱼类施用几种浓度的硝酸铅,24、48、72 和 96 小时后,记录死亡率(百分比)%。在整个实验期间,使用了七种不同浓度的硝酸铅来确定该物质的半数致死浓度,并评估其对鲤鱼死亡率的影响。硝酸铅的浓度介于 10 至 60 毫克/升之间,并测定了每个浓度的死亡率。结果发现,硝酸铅的 LC100 为 60 毫克/升。因此,本实验中使用的鲤鱼的急性 96 小时半数致死浓度值为 35 毫克/升(ppm)。
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