International Society for Extracellular Vesicles workshop. QuantitatEVs: Multiscale analyses, from bulk to single extracellular vesicle

Manuela Basso, Alessandro Gori, Caterina Nardella, Mari Palviainen, Marija Holcar, Ioannis Sotiropoulos, Sylwia Bobis-Wozowicz, Vito G. D'Agostino, Elena Casarotto, Yari Ciani, Shiro Suetsugu, Alice Gualerzi, Lorena Martin-Jaular, Daniela Boselli, Anna Kashkanova, Pietro Parisse, Lien Lippens, Martina Pagliuca, Martin Blessing, Roberto Frigerio, Thibaut Fourniols, Ana Meliciano, Anna Fietta, Paolo Vincenzo Fioretti, Karolina Soroczyńska, Silvia Picciolini, Amanda Salviano-Silva, Paolo Bergese, Davide Zocco, Marcella Chiari, Guido Jenster, Levi Waldron, Aleksandar Milosavljevic, John Nolan, Marco P. Monopoli, Kenneth W. Witwer, Benedetta Bussolati, Dolores Di Vizio, Juan Falcon Perez, Metka Lenassi, Marina Cretich, Francesca Demichelis
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Abstract

The “QuantitatEVs: multiscale analyses, from bulk to single vesicle” workshop aimed to discuss quantitative strategies and harmonized wet and computational approaches toward the comprehensive analysis of extracellular vesicles (EVs) from bulk to single vesicle analyses with a special focus on emerging technologies. The workshop covered the key issues in the quantitative analysis of different EV-associated molecular components and EV biophysical features, which are considered the core of EV-associated biomarker discovery and validation for their clinical translation. The in-person-only workshop was held in Trento, Italy, from January 31st to February 2nd, 2023, and continued in Milan on February 3rd with “Next Generation EVs,” a satellite event dedicated to early career researchers (ECR). This report summarizes the main topics and outcomes of the workshop.

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国际细胞外囊泡学会研讨会。定量EVs:从细胞外囊泡到单个细胞外囊泡的多尺度分析
细胞外囊泡定量分析:从体外囊泡到单囊泡的多尺度分析 "研讨会旨在讨论细胞外囊泡(EVs)从体外囊泡到单囊泡综合分析的定量策略、统一的湿法和计算方法,并特别关注新兴技术。研讨会涵盖了定量分析不同EV相关分子成分和EV生物物理特征的关键问题,这些问题被认为是EV相关生物标记物发现和临床转化验证的核心。该研讨会于 2023 年 1 月 31 日至 2 月 2 日在意大利特伦托举行,并于 2 月 3 日在米兰继续举行,同时还为早期职业研究人员(ECR)举办了 "下一代 EVs "卫星会议。本报告总结了研讨会的主要议题和成果。
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